Department of Advanced General Dentistry, College of Dentistry, Yonsei University, Seoul, Korea.
Division of Anatomy and Developmental Biology, Department of Oral Biology, Human Identification Research Center, Yonsei University College of Dentistry, Seoul, Korea.
Oral Dis. 2019 Apr;25(3):822-830. doi: 10.1111/odi.13037. Epub 2019 Feb 3.
To determine the synergistic effect of parathyroid hormone (PTH) [1-34] in combination with hyperbaric oxygen (HBO) on bone graft in a rat calvarial bone defect model under impaired osteogenic conditions.
Twenty-four rats were divided into three groups. Localized radiation with a single 12 Gy dose was administered to the calvaria. Four weeks after radiation, calvarial circular defects were created in the parietal bones. All defects were filled with biphasic calcium phosphate. After the bone graft, PTH [1-34] was injected subcutaneously, and HBO was administered. At 6 weeks after the bone graft, the rats were sacrificed, and specimens were harvested.
Histomorphometric evaluation showed that the percentage of new bone area was higher in the PTH and PTH/HBO groups than in the control group. The percent residual material area was decreased in the PTH/HBO group compared with the control group. The percentage blood vessel number was highest in the PTH group. Micro-CT evaluation showed that the new bone volume was highest in the PTH/HBO group. The residual material volume was lowest in the PTH/HBO group.
Within the limitations of this study, our data indicate that PTH combined with HBO may reverse radiation-induced impairment of bone healing.
在成骨条件受损的大鼠颅骨骨缺损模型中，确定甲状旁腺激素（PTH）[1-34]与高压氧（HBO）联合应用对骨移植物的协同作用。
将 24 只大鼠分为三组。颅骨局部给予单次 12Gy 剂量照射。照射后 4 周，在顶骨上制作颅骨圆形缺损。所有缺损均用双相磷酸钙填充。骨移植后，皮下注射 PTH[1-34]，并给予 HBO。骨移植后 6 周处死大鼠，采集标本。
组织形态计量学评估显示，PTH 组和 PTH/HBO 组的新骨面积百分比高于对照组。与对照组相比，PTH/HBO 组的残留材料面积减少。PTH 组的血管数量百分比最高。Micro-CT 评估显示，PTH/HBO 组的新骨体积最高。PTH/HBO 组的残留材料体积最低。
在本研究的限制范围内，我们的数据表明，PTH 联合 HBO 可能逆转辐射对骨愈合的损害。
