Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan; Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo 108-8639, Japan.
Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan; Department of Bioengineering, School of Engineering, The University of Tokyo, Hongo, Tokyo 113-8656, Japan.
J Pharm Sci. 2019 May;108(5):1686-1691. doi: 10.1016/j.xphs.2018.12.023. Epub 2019 Jan 8.
Cyclo olefin polymer (COP) is an attractive plastic because it has low protein adsorption despite its hydrophobic chemical structure. Here, the adsorption of model proteins to the COP was evaluated in comparison with a representative plastic, polystyrene (PSt), using reflectometry interference spectroscopy (RIfS) technology. The effects of different salts on adsorption were then examined. The adsorption of bovine serum albumin onto COP increased in the presence of kosmotropic salts, whereas adsorption of IgG increased in the presence of chaotropic salts. By contrast, the adsorption of these 2 proteins to PSt was unaffected by these Hofmeister salts. Langmuir-Freundlich model of COP adsorption suggested that the COP surface is more homogeneous for protein binding than the PSt surface. Furthermore, RIfS and sum frequency generation analyses indicated that water molecules bind more weakly to COP than to PSt. Our data propose a novel viewpoint of the way protein binds to COP surface that is different from the way it binds to PSt.
环烯烃聚合物（COP）是一种有吸引力的塑料，因为尽管其化学结构疏水，但它的蛋白质吸附率低。在这里，使用反射干涉光谱（RIfS）技术，将模型蛋白质对 COP 的吸附与代表性塑料聚苯乙烯（PSt）进行了比较评估。然后检查了不同盐对吸附的影响。在正离子盐的存在下，牛血清白蛋白（BSA）吸附到 COP 上的增加，而在离液盐的存在下，免疫球蛋白 G（IgG）的吸附增加。相比之下，这些 2 种蛋白质对 PSt 的吸附不受这些霍夫迈斯特盐的影响。COP 吸附的 Langmuir-Freundlich 模型表明，COP 表面对蛋白质结合的均匀性高于 PSt 表面。此外，RIfS 和和频产生分析表明，水分子与 COP 的结合比与 PSt 的结合弱。我们的数据提出了一种关于蛋白质与 COP 表面结合方式的新观点，与它与 PSt 结合的方式不同。
