Hua Xiaoliang, Chen Juan, Xu Yujie, Li Bing
Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Key Laboratory of Aquatic Biodiversity and Conservation of the Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Urology. 2019 Apr;126:209-216. doi: 10.1016/j.urology.2018.10.058. Epub 2019 Jan 8.
To investigate the efficacy of a combined dorsal plus ventral double tunica vaginalis graft for urethral reconstruction in a rabbit model through radiology and histopathology.
Thirty adult male New Zealand rabbits were randomly divided into 6 groups as follows (n = 5): normal, stricture, and experimental groups A, B, C, and D. In the stricture and experimental groups, the ventral urethra was incised longitudinally, and the dorsal and ventral urethral mucosa were partially removed. Then, 3 × 20 mm and 5 × 20 mm tunica vaginalis grafts were obtained to repair the dorsal and ventral urethral mucosa defects, respectively, and the spongiosum was closed in the experimental groups. The urethral defects were not repaired in the stricture group. The rabbits in experimental groups A, B, C, and D were sacrificed at 2 weeks, 4 weeks, 12 weeks, and 24 weeks postoperatively, respectively, and the rabbits in the stricture group were sacrificed at 4 weeks postoperatively. The urethra was harvested for histological analysis. Urethrography was performed before sacrifice in the stricture group and experimental groups B and D.
The retrograde urethrogram showed that all rabbits in experimental groups B and D had a patent urethra. Histological examination showed that the tunica vaginalis graft completely integrated into the urethra at 4 weeks postoperatively and transformed into a urinary pseudostratified epithelium at 12 weeks postoperatively.
Combined dorsal plus ventral double tunica vaginalis graft urethroplasty is a feasible technique for urethral reconstruction in a rabbit model.
通过放射学和组织病理学研究背侧加腹侧双层鞘膜移植在兔模型中进行尿道重建的疗效。
将30只成年雄性新西兰兔随机分为6组（n = 5）：正常组、狭窄组以及实验组A、B、C和D。在狭窄组和实验组中，纵向切开腹侧尿道，部分切除背侧和腹侧尿道黏膜。然后，分别获取3×20 mm和5×20 mm的鞘膜移植物来修复背侧和腹侧尿道黏膜缺损，实验组中海绵体予以闭合。狭窄组不修复尿道缺损。实验组A、B、C和D的兔分别在术后2周、4周、12周和24周处死，狭窄组的兔在术后4周处死。取出尿道进行组织学分析。在狭窄组以及实验组B和D处死前进行尿道造影。
逆行尿道造影显示实验组B和D的所有兔尿道通畅。组织学检查显示，鞘膜移植物在术后4周完全融入尿道，并在术后12周转化为尿路上皮假复层上皮。
背侧加腹侧双层鞘膜移植尿道成形术是兔模型中一种可行的尿道重建技术。
