Yoon Kee Dong, Chin Young-Won, Yang Min Hye, Kim Jinwoong
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, South Korea; College of Pharmacy, The Catholic University of Korea, Bucheon-si, Gyeonggi-do 420-743, South Korea.
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, South Korea; College of Pharmacy, Dongguk University-Seoul, Seoul 100-715, South Korea.
Food Chem. 2011 Nov 15;129(2):679-683. doi: 10.1016/j.foodchem.2011.05.005. Epub 2011 May 6.
High-speed countercurrent chromatography (HSCCC) was applied to separate two anti-ulcer flavonoids, eupatilin (5,7-dihydroxy-6,3',4'-trimethoxyflavone, 1) and jaceosidin (5,7,4'-trihydroxy-6,3'-dimethoxyflavone, 2), isolated from the ethanol, ethyl acetate and semi-purified ethanol extract of dietary wormwood (Artemisia princeps). The two phase solvent system comprising n-hexane-chloroform-methanol-water (2:5:5:2, v/v/v/v) was employed to HSCCC and the flow rate of mobile phase was optimised at 1.3ml/min. Utilising this method, the recovery rate and purity of eupatilin (1) and jaceosidin (2) separated from three Artemisia extracts were evaluated to be over 95% and 90%, respectively. These results suggest that countercurrent separation method can be applied to isolate two anti-ulcer flavonoids, eupatilin (1) and jaceosidin (2), selectively in one step, and to produce anti-ulcer flavonoid-enriched preparations.
高速逆流色谱法（HSCCC）用于分离两种抗溃疡类黄酮，即从食用艾草（Artemisia princeps）的乙醇、乙酸乙酯和半纯化乙醇提取物中分离得到的灯盏乙素（5,7-二羟基-6,3',4'-三甲氧基黄酮，1）和香叶木素（5,7,4'-三羟基-6,3'-二甲氧基黄酮，2）。采用正己烷-氯仿-甲醇-水（2:5:5:2，v/v/v/v）的两相溶剂体系进行高速逆流色谱分离，并将流动相流速优化至1.3ml/min。利用该方法，从三种艾草提取物中分离得到的灯盏乙素（1）和香叶木素（2）的回收率和纯度分别评估为超过95%和90%。这些结果表明，逆流分离法可用于一步选择性地分离两种抗溃疡类黄酮，即灯盏乙素（1）和香叶木素（2），并制备富含抗溃疡类黄酮的制剂。
