Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland.
Int J Mol Sci. 2019 Jan 9;20(2):235. doi: 10.3390/ijms20020235.
Auxins are postulated to be one of the pivotal factors in nodulation. However, their transporters in , the model species for the study of the development of determinate-type root nodules, have been scarcely described so far, and thus their role in nodulation has remained unknown. Our research is the first focusing on polar auxin transporters in . We analyzed and compared expression of in 20 days post rhizobial inoculation (dpi) and 54 dpi root nodules of by real-time quantitative polymerase chain reaction (qPCR) along with the histochemical β-glucuronidase () reporter gene assay in transgenic hairy roots. The results indicate that are essential during root nodule development since they are predominantly expressed in the primordia and young, developing nodules. However, along with differentiation, expression levels of several decreased and occurred particularly in the nodule vascular bundles, especially in connection with the root's stele. Moreover, our study demonstrated the importance of both polar auxin transport and auxin intracellular homeostasis during root nodule development and differentiation.
植物生长素被认为是根瘤形成的关键因素之一。然而，到目前为止，关于其在 （根瘤菌共生结瘤的模式物种）中的转运体，研究甚少，因此其在结瘤过程中的作用尚不清楚。我们的研究首次聚焦于 中的极性生长素转运体。我们通过实时定量聚合酶链反应（qPCR）和转 hairy 根中的组织化学β-葡萄糖醛酸酶（GUS）报告基因分析，比较了接种根瘤菌 20 天（dpi）和 54 dpi 根瘤中 的表达。结果表明， 在根瘤发育过程中是必需的，因为它们主要在原基和年轻的、发育中的根瘤中表达。然而，随着分化，几个 的表达水平下降，特别是在根瘤维管束中，特别是与根的中柱相连。此外，我们的研究还证明了极性生长素运输和生长素细胞内稳态在 根瘤发育和分化过程中的重要性。
