Cell Signal Transduction Laboratory, Bioinformatics Center, Department of Preventive Medicine, Institute of Biomedical Informatics, School of Basic Medical Sciences, Henan University, Kaifeng 475004, China.
Public Health Research Institute at New Jersey Medical School, Rutgers State University of New Jersey, 225 Warren Street, Newark, NJ 07103, USA.
Genes (Basel). 2019 Jan 10;10(1):36. doi: 10.3390/genes10010036.
Keratin 8 (KRT8), a type II basic intermediate filament (IF) protein, is essential for the development and metastasis of various cancers. In this study, by analyzing RNA-seq data from the Cancer Genome Atlas (TCGA)-lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC), we have determined the expression profile of , and assessed its prognostic significance and the possible mechanism underlying the dysregulation. Our results showed that mRNA expression was significantly up-regulated in both LUAD and LUSC tissues compared with normal lung tissues. The high expression group for LUAD patients significantly reduced overall survival (OS) and recurrence-free survival (RFS). Univariate and multivariate analysis revealed that expression was an independent prognostic indicator for poor OS and RFS in LUAD patients. However, expression had no prognostic value in terms of OS and RFS for LUSC. By exploring DNA copy number alterations (CNAs) of the gene in LUAD, we found that DNA low copy gain (+1 and +2) was associated with elevated mRNA expression. From the above findings, we have deduced that is aberrantly expressed in LUAD tissues and that its expression might independently predict poor OS and RFS for LUAD patients, but not for LUSC patients.
角蛋白 8（KRT8），一种 II 型基本中间丝（IF）蛋白，对各种癌症的发生和转移至关重要。在这项研究中，我们通过分析癌症基因组图谱（TCGA）-肺腺癌（LUAD）和肺鳞状细胞癌（LUSC）的 RNA-seq 数据，确定了 的表达谱，并评估了其预后意义和失调的可能机制。我们的结果表明，与正常肺组织相比，KRT8 mRNA 在 LUAD 和 LUSC 组织中均显著上调。LUAD 患者中高 KRT8 表达组的总生存（OS）和无复发生存（RFS）显著降低。单因素和多因素分析表明，KRT8 表达是 LUAD 患者 OS 和 RFS 的独立预后指标。然而，KRT8 表达对 LUSC 患者的 OS 和 RFS 无预后价值。通过探索 LUAD 中 KRT8 基因的 DNA 拷贝数改变（CNAs），我们发现 DNA 低拷贝增益（+1 和+2）与 KRT8 mRNA 表达升高相关。根据上述发现，我们推断 KRT8 在 LUAD 组织中异常表达，其表达可能独立预测 LUAD 患者的 OS 和 RFS 不良，但对 LUSC 患者则不然。
