Erickson G F, Magoffin D A, Unger M, Allen W R, Dulbecco R
School of Medicine, University of California, San Diego, La Jolla 92093.
Mol Cell Endocrinol. 1988 Dec;60(2-3):177-87. doi: 10.1016/0303-7207(88)90177-3.
A major problem in ovarian physiology is the lack of conveniently quantifiable markers of atresia. Towards this end, we identified a monoclonal antibody (anti-OA-2) that selectively recognizes granulosa cells in atretic follicles. When cryostat sections of rat ovaries were incubated with anti-OA-2, granulosa cells in atretic follicles showed intense immunofluorescent labeling. In contrast, no anti-OA-2 immunoreactivity was observed in the granulosa of the healthy follicles. The amount of anti-OA-2 binding was significantly enhanced when atresia was stimulated by treatment with human chorionic gonadotropin, testosterone, or estrogen withdrawal. The results of immunoprecipitation and Western blot analyses indicated that the OA-2 antigen is a 39 kDa protein which is actively synthesized by the granulosa during atresia. The 39 kDa protein is localized at or near the inner surface of the plasma membrane. We conclude that the anti-OA-2 monoclonal will prove useful as a convenient analytical tool to study the regulation of granulosa atresia.
卵巢生理学中的一个主要问题是缺乏便于量化的闭锁标记物。为此，我们鉴定出一种单克隆抗体（抗OA-2），它能选择性识别闭锁卵泡中的颗粒细胞。当用抗OA-2孵育大鼠卵巢的冷冻切片时，闭锁卵泡中的颗粒细胞呈现强烈的免疫荧光标记。相比之下，在健康卵泡的颗粒细胞中未观察到抗OA-2免疫反应性。当通过人绒毛膜促性腺激素、睾酮或雌激素撤药刺激闭锁时，抗OA-2结合量显著增加。免疫沉淀和蛋白质免疫印迹分析结果表明，OA-2抗原是一种39 kDa的蛋白质，在闭锁过程中由颗粒细胞主动合成。这种39 kDa的蛋白质定位于质膜内表面或其附近。我们得出结论，抗OA-2单克隆抗体将被证明是研究颗粒细胞闭锁调节的一种方便的分析工具。
