舞毒蛾核型多角体病毒的物理图谱和多角体蛋白基因序列

Physical map and polyhedrin gene sequence of Lymantria dispar nuclear polyhedrosis virus.

作者信息

Smith I R, van Beek N A, Podgwaite J D, Wood H A

机构信息

Boyce Thompson Institute, Ithaca, NY 14853.

出版信息

Gene. 1988 Nov 15;71(1):97-105. doi: 10.1016/0378-1119(88)90081-9.

DOI:10.1016/0378-1119(88)90081-9

PMID:3063616

Abstract

Restriction maps of the 166.6-kb genome of Lymantria dispar multiply-enveloped nuclear polyhedrosis virus (LdMNPV clone g) were constructed for BamHI, BglII, EcoRI, EcoRV, HindIII and KpnI, using cosmid pVK102 and pBluescript vectors. Southern hybridizations indicated that the LdMNPV genome contains five dispersed regions of intragenomic sequence homology. The polyhedrin gene of LdMNPV was located within BglII-E and the sequence of the 735-nucleotide (nt) coding region and 678 nt of flanking DNA was determined. A conserved 14-nt sequence, associated with transcriptional start points in other polyhedrins, was identified at 44 to 57 nt upstream from the start codon. The deduced polyhedrin amino acid (aa) sequence showed a high degree of homology with a previously determined protein sequence for LdMNPV polyhedrin (89%) and with deduced amino acid sequences for three other MNPV polyhedrins (74%). Optimal alignment of the four sequences indicated that LdMNPV polyhedrin possesses a single aa insertion at residue 4 and a single aa deletion at residue 164.

摘要

利用黏粒pVK102和pBluescript载体构建了舞毒蛾多粒包埋核型多角体病毒（LdMNPV克隆g）166.6 kb基因组的BamHI、BglII、EcoRI、EcoRV、HindIII和KpnI限制酶图谱。Southern杂交表明，LdMNPV基因组包含五个基因组内序列同源性的分散区域。LdMNPV的多角体蛋白基因位于BglII-E内，并测定了735个核苷酸（nt）编码区和678 nt侧翼DNA的序列。在起始密码子上游44至57 nt处鉴定出一个与其他多角体蛋白转录起始点相关的保守14 nt序列。推导的多角体蛋白氨基酸（aa）序列与先前确定的LdMNPV多角体蛋白的蛋白质序列具有高度同源性（89%），与其他三种MNPV多角体蛋白的推导氨基酸序列具有高度同源性（74%）。这四个序列的最佳比对表明，LdMNPV多角体蛋白在第4位残基处有一个单aa插入，在第164位残基处有一个单aa缺失。