Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Inner Mongolia Autonomous Region Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science,Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Mol Reprod Dev. 2019 Mar;86(3):342-350. doi: 10.1002/mrd.23110. Epub 2019 Jan 18.
The low efficiency of in vitro embryo production is associated with oxidative stress induced by suboptimal culture conditions. p66Shc is a 66-kDa protein of the ShcA (Src homologous-collagen homolog) adaptor protein family, which is involved in signaling pathways involved in oxidative stress regulation, apoptosis induction, and aging. However, the functional role of p66Shc during the preimplantation development of sheep embryos is not understood. Our results showed that early-cleavage (≤28 hr) embryos had a higher developmental potential than late-cleavage (>28 hr) embryos. The poor quality of these late-cleavage embryos was associated with increased the transcripts and protein of p66Shc and decreased mitochondrial activity. In addition, exogenous hydrogen peroxide-induced oxidative stress significantly increased p66Shc protein abundance and suppressed embryonic development, which was ameliorated by antioxidant treatment. Notably, oxidative stress induced the nuclear localization of p66Shc and phosphorylated (Ser-36) p66Shc. Collectively, these observations suggest that p66Shc may be playing an important role in the regulation of oxidative stress during the preimplantation development of sheep embryos.
体外胚胎生产效率低下与次优培养条件引起的氧化应激有关。p66Shc 是 ShcA（Src 同源胶原同系物）衔接蛋白家族的一种 66kDa 蛋白，参与涉及氧化应激调节、细胞凋亡诱导和衰老的信号通路。然而，p66Shc 在绵羊胚胎植入前发育过程中的功能作用尚不清楚。我们的研究结果表明，早期分裂（≤28 小时）胚胎比晚期分裂（>28 小时）胚胎具有更高的发育潜能。这些晚期分裂胚胎的质量较差，与 p66Shc 的转录本和蛋白增加以及线粒体活性降低有关。此外，外源性过氧化氢诱导的氧化应激显著增加了 p66Shc 蛋白的丰度，抑制了胚胎发育，抗氧化剂处理可改善这种情况。值得注意的是，氧化应激诱导 p66Shc 的核定位和磷酸化（Ser-36）p66Shc。综上所述，这些观察结果表明，p66Shc 可能在绵羊胚胎植入前发育过程中氧化应激的调节中发挥重要作用。
