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重组人骨形态发生蛋白-2与低强度激光照射对双膦酸盐处理的成骨细胞的联合作用。

Combined effect of recombinant human bone morphogenetic protein-2 and low level laser irradiation on bisphosphonate-treated osteoblasts.

作者信息

Jeong Seok-Young, Hong Ji-Un, Song Jae Min, Kim In Ryoung, Park Bong Soo, Kim Chul Hoon, Shin Sang Hun

机构信息

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Korea.

Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan, Korea.

出版信息

J Korean Assoc Oral Maxillofac Surg. 2018 Dec;44(6):259-268. doi: 10.5125/jkaoms.2018.44.6.259. Epub 2018 Dec 28.

DOI:10.5125/jkaoms.2018.44.6.259
PMID:30637239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6327014/
Abstract

OBJECTIVES

The purpose of this study was to evaluate the synergic effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) and low-level laser therapy (LLLT) on bisphosphonate-treated osteoblasts.

MATERIALS AND METHODS

Human fetal osteoblast cells (hFOB 1.19) were cultured with 100 µM alendronate. Low-level Ga-Al-As laser alone or with 100 ng/mL rhBMP-2 was then applied. Cell viability was measured with MTT assay. The expression levels of receptor activator of nuclear factor kappa-B ligand (RANKL), macrophage colony-stimulating factor (M-CSF), and osteoprotegerin (OPG) were analyzed for osteoblastic activity inducing osteoclastic activity. Collagen type and transforming growth factor beta-1 were also evaluated for bone matrix formation.

RESULTS

The results showed that rhBMP-2 and LLLT had a synergic effect on alendronate-treated osteoblasts for enhancing osteoblastic activity and bone matrix formation. Between rhBMP-2 and LLLT, rhBMP-2 exhibited a greater effect, but did not show a significant difference.

CONCLUSION

rhBMP-2 and LLLT have synergic effects on bisphosphonate-treated osteoblasts through enhancement of osteoblastic activity and bone formation activity.

摘要

目的

本研究旨在评估重组人骨形态发生蛋白-2（rhBMP-2）与低强度激光疗法（LLLT）对经双膦酸盐处理的成骨细胞的协同作用。

材料与方法

将人胎儿成骨细胞（hFOB 1.19）与100 μM阿仑膦酸钠一起培养。然后单独应用低强度镓铝砷激光或与100 ng/mL rhBMP-2联合应用。采用MTT法测定细胞活力。分析核因子κB受体活化因子配体（RANKL）、巨噬细胞集落刺激因子（M-CSF）和骨保护素（OPG）的表达水平，以评估诱导破骨细胞活性的成骨细胞活性。还评估了I型胶原蛋白和转化生长因子β-1以分析骨基质形成情况。

结果

结果表明，rhBMP-2和LLLT对经阿仑膦酸钠处理的成骨细胞具有协同作用，可增强成骨细胞活性和骨基质形成。在rhBMP-2和LLLT之间，rhBMP-2表现出更大的作用，但差异不显著。

结论

rhBMP-2和LLLT通过增强成骨细胞活性和骨形成活性，对经双膦酸盐处理的成骨细胞具有协同作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/ee9e978e48fd/jkaoms-44-259-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/958b03f5b84c/jkaoms-44-259-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/86af84dbc969/jkaoms-44-259-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/8e5754775d5c/jkaoms-44-259-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/76d77bd8e96b/jkaoms-44-259-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/30951fc6e949/jkaoms-44-259-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/e427825209c9/jkaoms-44-259-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/d6adf8dcd93e/jkaoms-44-259-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/ee9e978e48fd/jkaoms-44-259-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/958b03f5b84c/jkaoms-44-259-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/86af84dbc969/jkaoms-44-259-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/8e5754775d5c/jkaoms-44-259-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/76d77bd8e96b/jkaoms-44-259-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/30951fc6e949/jkaoms-44-259-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/e427825209c9/jkaoms-44-259-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/d6adf8dcd93e/jkaoms-44-259-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69c7/6327014/ee9e978e48fd/jkaoms-44-259-g008.jpg

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