一种高通量机器人组装三维血管组织的方法。
A Method for High-Throughput Robotic Assembly of Three-Dimensional Vascular Tissue.
机构信息
Robotics Engineering Program, Worcester Polytechnic Institute, Worcester, Massachusetts.
Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, Massachusetts.
出版信息
Tissue Eng Part A. 2019 Sep;25(17-18):1251-1260. doi: 10.1089/ten.TEA.2018.0288. Epub 2019 Aug 9.
Abstract
Self-assembled tissues have potential to serve both as implantable grafts and as tools for disease modeling and drug screening. For these applications, tissue production must ultimately be scaled-up and automated. Limited technologies exist for precisely manipulating self-assembled tissues, which are fragile early in culture. Here, we presented a method for automatically stacking self-assembled smooth muscle cell rings onto mandrels, using a custom-designed well plate and robotic punch system. Rings then fuse into tissue-engineered blood vessels (TEBVs). This is a critical step toward automating TEBV production that may be applied to other tubular tissues as well.
摘要
自组装组织具有作为可植入移植物以及用于疾病建模和药物筛选工具的潜力。对于这些应用，组织生产最终必须规模化和自动化。目前用于精确操作自组装组织的技术有限，因为这些组织在培养早期非常脆弱。在这里，我们使用定制设计的孔板和机器人打孔系统，提出了一种将自组装平滑肌细胞环自动堆叠到心轴上的方法。然后，环融合成组织工程血管（TEBV）。这是朝着自动化 TEBV 生产迈出的关键一步，也可应用于其他管状组织。
相似文献
1
A Method for High-Throughput Robotic Assembly of Three-Dimensional Vascular Tissue.一种高通量机器人组装三维血管组织的方法。
Tissue Eng Part A. 2019 Sep;25(17-18):1251-1260. doi: 10.1089/ten.TEA.2018.0288. Epub 2019 Aug 9.
2
Implantable tissue-engineered blood vessels from human induced pluripotent stem cells.源自人类诱导多能干细胞的可植入组织工程血管
Biomaterials. 2016 Sep;102:120-9. doi: 10.1016/j.biomaterials.2016.06.010. Epub 2016 Jun 14.
3
Assembly of Tissue-Engineered Blood Vessels with Spatially Controlled Heterogeneities.组织工程血管的具有空间控制异质性的组装。
Tissue Eng Part A. 2018 Oct;24(19-20):1492-1503. doi: 10.1089/ten.TEA.2017.0492. Epub 2018 Aug 20.
4
Rapid Self-Assembly of Bioengineered Cardiovascular Bypass Grafts From Scaffold-Stabilized, Tubular Bilevel Cell Sheets.生物工程心血管旁路移植物的快速自组装：支架稳定的双层管状细胞片。
Circulation. 2018 Nov 6;138(19):2130-2144. doi: 10.1161/CIRCULATIONAHA.118.035231.
5
Vascular smooth muscle enhances functionality of tissue-engineered blood vessels in vivo.血管平滑肌增强了体内组织工程血管的功能。
J Vasc Surg. 2011 Feb;53(2):426-34. doi: 10.1016/j.jvs.2010.07.054.
6
A novel single-step self-assembly approach for the fabrication of tissue-engineered vascular constructs.一种用于构建组织工程血管构建体的新型单步自组装方法。
Tissue Eng Part A. 2010 May;16(5):1737-47. doi: 10.1089/ten.TEA.2009.0313.
7
Manipulation of remodeling pathways to enhance the mechanical properties of a tissue engineered blood vessel.调控重塑途径以增强组织工程血管的力学性能。
J Biomech Eng. 2002 Dec;124(6):724-33. doi: 10.1115/1.1519278.
8
Mechano-active tissue engineering of vascular smooth muscle using pulsatile perfusion bioreactors and elastic PLCL scaffolds.使用脉动灌注生物反应器和弹性聚（L-丙交酯-co-ε-己内酯）支架对血管平滑肌进行机械活性组织工程构建
Biomaterials. 2005 Apr;26(12):1405-11. doi: 10.1016/j.biomaterials.2004.04.036.
9
Cellular Self-Assembly with Microsphere Incorporation for Growth Factor Delivery Within Engineered Vascular Tissue Rings.用于在工程化血管组织环内递送生长因子的含微球细胞自组装
Tissue Eng Part A. 2017 Feb;23(3-4):143-155. doi: 10.1089/ten.TEA.2016.0260. Epub 2016 Dec 6.
10
A collagen/smooth muscle cell-incorporated elastic scaffold for tissue-engineered vascular grafts.用于组织工程血管移植物的胶原蛋白/平滑肌细胞掺入弹性支架。
J Biomater Sci Polym Ed. 2009;20(11):1645-60. doi: 10.1163/156856208X386237.
引用本文的文献
1
Tetanus-driven biohybrid multijoint robots powered by muscle rings with enhanced contractile force.由具有增强收缩力的肌肉环驱动的破伤风驱动生物混合多关节机器人。
Sci Adv. 2025 Jul 18;11(29):eadu9962. doi: 10.1126/sciadv.adu9962. Epub 2025 Jul 16.
2
3D Tissue-Engineered Vascular Drug Screening Platforms: Promise and Considerations.3D组织工程血管药物筛选平台：前景与考量
Front Cardiovasc Med. 2022 Mar 4;9:847554. doi: 10.3389/fcvm.2022.847554. eCollection 2022.
本文引用的文献
1
A Modular Strategy to Engineer Complex Tissues and Organs.一种构建复杂组织和器官的模块化策略。
Adv Sci (Weinh). 2018 Feb 14;5(5):1700402. doi: 10.1002/advs.201700402. eCollection 2018 May.
2
Assembly of Tissue-Engineered Blood Vessels with Spatially Controlled Heterogeneities.组织工程血管的具有空间控制异质性的组装。
Tissue Eng Part A. 2018 Oct;24(19-20):1492-1503. doi: 10.1089/ten.TEA.2017.0492. Epub 2018 Aug 20.
3
Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds.使用3D打印模具制造用于细胞接种和组织环自组装的定制琼脂糖孔。
J Vis Exp. 2018 Apr 2(134):56618. doi: 10.3791/56618.
4
Automated 3D bioassembly of micro-tissues for biofabrication of hybrid tissue engineered constructs.自动化 3D 生物组装微组织用于混合组织工程构建体的生物制造。
Biofabrication. 2018 Jan 12;10(2):024103. doi: 10.1088/1758-5090/aa9ef1.
5
Fabrication and characterization of electrospun polycaprolactone and gelatin composite cuffs for tissue engineered blood vessels.静电纺丝聚己内酯和明胶复合套管的制备及性能表征用于组织工程血管
J Biomed Mater Res B Appl Biomater. 2018 Feb;106(2):817-826. doi: 10.1002/jbm.b.33871. Epub 2017 Apr 6.
6
Principles of the Kenzan Method for Robotic Cell Spheroid-Based Three-Dimensional Bioprinting<sup/>.基于细胞球体的机器人三维生物打印的 Kenzan 方法的原理<sup/>。
Tissue Eng Part B Rev. 2017 Jun;23(3):237-244. doi: 10.1089/ten.TEB.2016.0322. Epub 2017 Jan 3.
7
Cellular Self-Assembly with Microsphere Incorporation for Growth Factor Delivery Within Engineered Vascular Tissue Rings.用于在工程化血管组织环内递送生长因子的含微球细胞自组装
Tissue Eng Part A. 2017 Feb;23(3-4):143-155. doi: 10.1089/ten.TEA.2016.0260. Epub 2016 Dec 6.
8
The bio-gripper: a fluid-driven micro-manipulator of living tissue constructs for additive bio-manufacturing.生物夹爪：一种用于添加生物制造的液体驱动的活组织构建体微操纵器。
Biofabrication. 2016 May 25;8(2):025015. doi: 10.1088/1758-5090/8/2/025015.
9
State-of-the-Art Review of 3D Bioprinting for Cardiovascular Tissue Engineering.心血管组织工程3D生物打印技术的最新综述
Ann Biomed Eng. 2017 Jan;45(1):195-209. doi: 10.1007/s10439-016-1607-5. Epub 2016 Apr 11.
10
Scaffold-Free Tubular Tissues Created by a Bio-3D Printer Undergo Remodeling and Endothelialization when Implanted in Rat Aortae.生物3D打印机制造的无支架管状组织植入大鼠主动脉后会发生重塑和内皮化。
PLoS One. 2015 Sep 1;10(9):e0136681. doi: 10.1371/journal.pone.0136681. eCollection 2015.
Zotero 插件