Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119, India.
Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119, India.
Enzyme Microb Technol. 2019 Mar;122:90-100. doi: 10.1016/j.enzmictec.2018.12.008. Epub 2018 Dec 21.
The aim of this study is to enhance the production of industrially valuable xylanase and pectinase enzymes in short duration, using agrowaste extracted substrates. Conventional cum statistical multifactor analysis approaches were used in order to evaluate the effect of crude extracted substrates, supplemented for the production of xylanase-pectinase enzymes. Incorporation of crude extracted xylan (1.2 mg/ml of inoculum) and pectin (4.8 mg/ml of inoculum) substrates in inoculum resulted in maximal xylanase (320 ± 15) and pectinase titre (90 ± 8) after 48 h, using 2% wheat bran and 2% citrus peel in production medium with 48 h of fermentation time, with one variable factor at a time approach. The best condition obtained after performing statistical multifactor interaction analysis includes 5.50 mg/ml of pectin in inoculum,1.50 mg/ml of xylan in inoculum, wheat bran 3%, temperature 37.5 °C, time 48 h, 7 mg/ml of pectin in production medium, peptone 1.05%, inoculum size 2% and inoculum age of 20 h, with alkaline xylanase activity of 415.22 ± 18.50 IU/ml and alkaline pectinase activity of 109.10 ± 8.80 IU/ml. Activity of different pectinolytic enzymes per ml was also calculated, with 18.98 IU of exo-polymethylgalacturonase, 0.14 IU of endo-polymethylgalacturonase, 80 IU of exo-polygalacturonase, 0.28 IU of endo-polygalacturonase, 1.42 IU of polymethylgalacturonate lyase, 1.47 IU of polygalacturonate lyase, 0.15 IU of pectin esterase. This is the first report mentioning the utilization of crude extracted xylan and extracted pectin in inoculum to get the increment in the activity of both alkaline xylanase-pectinase enzymes simultaneously under short submerged fermentation cycle.
本研究旨在通过利用农业废弃物提取的基质，在短时间内提高工业上有价值的木聚糖酶和果胶酶的产量。采用常规和统计多因素分析方法来评估粗提基质对木聚糖酶-果胶酶生产的影响。在接种物中加入粗提木聚糖（1.2mg/ml 接种物）和果胶（4.8mg/ml 接种物）基质，在发酵 48 小时后，使用 2%的麦麸和 2%的柑橘皮作为生产培养基，可获得最大的木聚糖酶（320±15）和果胶酶效价（90±8），采用单变量因子分析方法。通过执行统计多因素相互作用分析后得到的最佳条件包括：接种物中果胶为 5.50mg/ml、木聚糖为 1.50mg/ml、麦麸为 3%、温度为 37.5°C、时间为 48 小时、生产培养基中果胶为 7mg/ml、蛋白胨为 1.05%、接种物量为 2%、接种物龄为 20 小时，碱性木聚糖酶活性为 415.22±18.50IU/ml，碱性果胶酶活性为 109.10±8.80IU/ml。还计算了每毫升不同果胶酶的活性，其中外切多聚半乳糖醛酸酶为 18.98IU、内切多聚半乳糖醛酸酶为 0.14IU、外切聚半乳糖醛酸酶为 80IU、内切聚半乳糖醛酸酶为 0.28IU、多聚半乳糖醛酸裂解酶为 1.42IU、聚半乳糖醛酸裂解酶为 1.47IU、果胶酯酶为 0.15IU。这是首次报道在短时间浸没发酵周期内，利用粗提木聚糖和提取果胶在接种物中同时提高碱性木聚糖酶-果胶酶活性。
