溃疡性结肠炎患者的肠道黏膜愈合与紧密连接蛋白表达的相关性。

Correlation of Intestinal Mucosal Healing and Tight Junction Protein Expression in Ulcerative Colitis Patients.

机构信息

Department of Gastroenterology, Shengjing Hospital of China Medical University, Shenyang Liaoning Province, China.

出版信息

Am J Med Sci. 2019 Mar;357(3):195-204. doi: 10.1016/j.amjms.2018.11.011. Epub 2018 Nov 27.

DOI:10.1016/j.amjms.2018.11.011

PMID:30638599

Abstract

BACKGROUND

The aim of this study was to investigate the relationship between intestinal mucosal healing and tight junction (TJ) protein expression in patients with ulcerative colitis (UC).

MATERIALS AND METHODS

A total of 40 patients with UC were included as an experimental group and UC disease activity was evaluated using the Mayo clinic score (MCS) and 8 patients with normal distal colon served as the control group. The expression of TJ proteins including occludin, ZO-1 and claudin-2 were determined by immunohistochemistry and their correlation with clinical characteristics were also analyzed.

RESULTS

Statistically significant differences regarding the MCS and Mayo endoscopic subscore (MES) were observed in both groups (P < 0.01). The Geboes index was significantly increased in patients with active UC compared to patients with quiescent UC and normal controls (P < 0.01). Patients with active and quiescent UC had upregulated expression of claudin-2 and reduced expression of occludin and ZO-1 compared to those of normal controls. The expression of ZO-1 was significantly higher in patients with quiescent UC with mucosa healing (P < 0.05) compared with those without mucosal healing. The expression of ZO-1 and occludin was negatively correlated with MCS, MES, Geboes, C-reaction protein and erythrocyte sedimentation rate. The expression of claudin-2 was positively correlated with MCS, MES, Geboes, C-reaction protein and erythrocyte sedimentation rate.

CONCLUSIONS

These findings suggest that TJ proteins play a crucial role in mucosal healing, and may be a potential marker of response when evaluating therapeutic interventions.

摘要

背景

本研究旨在探讨溃疡性结肠炎（UC）患者肠黏膜愈合与紧密连接（TJ）蛋白表达的关系。

材料与方法

共纳入 40 例 UC 患者作为实验组，采用Mayo 临床评分（MCS）评估 UC 疾病活动度，另选 8 例远端结肠正常者作为对照组。采用免疫组化法检测 TJ 蛋白（occludin、ZO-1 和 claudin-2）的表达，并分析其与临床特征的相关性。

结果

两组的 MCS 和 Mayo 内镜评分（MES）均有统计学差异（P<0.01）。活动期 UC 的 Geboes 指数明显高于缓解期 UC 和对照组（P<0.01）。与对照组相比，活动期和缓解期 UC 患者的 claudin-2 表达上调，occludin 和 ZO-1 表达下调。黏膜愈合的缓解期 UC 患者的 ZO-1 表达明显高于无黏膜愈合的患者（P<0.05）。ZO-1 和 occludin 的表达与 MCS、MES、Geboes、C 反应蛋白和红细胞沉降率呈负相关，claudin-2 的表达与 MCS、MES、Geboes、C 反应蛋白和红细胞沉降率呈正相关。