Laboratory of Plant Physiology "Coaracy M. Franco", Center R&D in Ecophysiology and Biophysics, Agronomic Institute (IAC), Campinas, SP, Brazil.
Center for Natural and Human Sciences, Federal University of ABC, Santo André, SP, Brazil.
Nitric Oxide. 2019 Mar 1;84:38-44. doi: 10.1016/j.niox.2019.01.004. Epub 2019 Jan 9.
The entrapment of NO donors in nanomaterials has emerged as a strategy to protect these molecules from rapid degradation, allowing a more controlled release of NO and prolonging its effect. On the other hand, we have found beneficial effects of S-nitrosoglutathione (GSNO) - a NO donor - supplying to sugarcane plants under water deficit. Here, we hypothesized that GSNO encapsulated into nanoparticles would be more effective in attenuating the effects of water deficit on sugarcane plants as compared to the supplying of GSNO in its free form. The synthesis and characterization of chitosan nanoparticles containing GSNO were also reported. Sugarcane plants were grown in nutrient solution, and then subjected to the following treatments: control (well-hydrated); water deficit (WD); WD + GSNO sprayed in its free form (WDG) or encapsulated (WDG-NP). In general, both GSNO forms attenuated the effects of water deficit on sugarcane plants. However, the encapsulation of this donor into chitosan nanoparticles caused higher photosynthetic rates under water deficit, as compared to plants supplied with free GSNO. The root/shoot ratio was also increased when encapsulated GSNO was supplied, indicating that delayed release of NO improves drought tolerance of sugarcane plants. Our results provide experimental evidence that nanotechnology can be used for enhancing NO-induced benefits for plants under stressful conditions, alleviating the negative impact of water deficit on plant metabolism and increasing biomass allocation to root system.
氮氧化物供体被捕获在纳米材料中，已成为保护这些分子免受快速降解的一种策略，使 NO 更可控地释放，并延长其作用时间。另一方面，我们发现 S-亚硝基谷胱甘肽（GSNO）——一种 NO 供体——在甘蔗缺水时供应会产生有益的效果。在这里，我们假设与以游离形式供应 GSNO 相比，将 GSNO 封装在纳米颗粒中会更有效地减轻缺水对甘蔗植物的影响。我们还报告了含有 GSNO 的壳聚糖纳米颗粒的合成和表征。甘蔗植物在营养液中生长，然后进行以下处理：对照（充分浇水）；缺水（WD）；WD+以游离形式喷洒的 GSNO（WDG）或封装（WDG-NP）。一般来说，两种 GSNO 形式都减轻了缺水对甘蔗植物的影响。然而，与用游离 GSNO 供应的植物相比，将这种供体封装在壳聚糖纳米颗粒中会导致在缺水条件下更高的光合作用速率。当供应封装的 GSNO 时，根/茎比也增加了，这表明 NO 的延迟释放可以提高甘蔗植物的耐旱性。我们的研究结果提供了实验证据，表明纳米技术可用于增强植物在胁迫条件下由 NO 诱导的益处，减轻缺水对植物新陈代谢的负面影响，并增加生物量分配到根系。
