Cátedra CONACYT, Laboratory of Aquatic Toxicology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, AV. Wilfrido Massieu s/n y cerrada Manuel Stampa, Col. Industrial Vallejo., Ciudad de México, CP, 07700, Mexico; Laboratory of Aquatic Toxicology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, AV. Wilfrido Massieu s/n y cerrada Manuel Stampa, Col. Industrial Vallejo., Ciudad de México, CP, 07700, Mexico.
Laboratory of Aquatic Toxicology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, AV. Wilfrido Massieu s/n y cerrada Manuel Stampa, Col. Industrial Vallejo., Ciudad de México, CP, 07700, Mexico.
Environ Toxicol Pharmacol. 2019 Feb;66:96-103. doi: 10.1016/j.etap.2018.12.017. Epub 2018 Dec 21.
The aim of this research was to determine the bioconcentration factor and if subacute exposure to carbamazepine (2 mg L) modifies the oxidative state of liver, gills and brain of Cyprinus carpio. This was measured through the following biomarkers: hydroperoxide and protein carbonyl content, lipid peroxidation degree, as well as superoxide dismutase, catalase and glutathione peroxidase activity. Carbamazepine concentration in carp's tissue was also determined by liquid chromatography with a diode arrangement detector. An increase in lipid peroxidation degree, hydroperoxide and protein carbonyl content, and a decrease in the activity of the antioxidant enzymes (P < 0.05) with respect to control was observed. Also, there is an increase in the concentration of carbamazepina present in the organs with respect to the water in the system, which denotes bioconcentration of the drug. In conclusion, carbamazepine is bioconcentrated and produces oxidative stress on the common carp (C. carpio).
本研究旨在测定生物浓缩因子，以及亚急性暴露于卡马西平（2mg/L）是否会改变鲤鱼肝脏、鳃和大脑的氧化状态。这是通过以下生物标志物来衡量的：过氧化氢和蛋白质羰基含量、脂质过氧化程度以及超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性。鲤鱼组织中的卡马西平浓度也通过二极管排列检测器的液相色谱法进行了测定。与对照组相比，观察到脂质过氧化程度、过氧化氢和蛋白质羰基含量的增加，以及抗氧化酶活性的降低（P<0.05）。此外，与系统中的水相比，器官中卡马西平的浓度增加，这表示药物的生物浓缩。总之，卡马西平在鲤鱼（C. carpio）体内被生物浓缩，并产生氧化应激。
