Medical College of Georgia, Augusta University, Augusta, Georgia.
Division of Dermatology, Loyola University Medical Center, Maywood, Illinois.
Dermatol Surg. 2019 Jun;45(6):768-771. doi: 10.1097/DSS.0000000000001747.
The Mohs histotechnologist (MH) performs tissue preparation, sectioning, and staining, which are critical tasks in ensuring a successful Mohs micrographic surgery (MMS).
To assess current norms in MH training, practice setting, and utilization of specific histologic techniques.
A 16-question survey was created and distributed using Survey Monkey to all members of the American Society for Mohs Histotechnology.
Response rate was 30%. Most MHs received on-the-job training from other MHs or the Mohs surgeon. Mohs histotechnologists largely performed tasks related to tissue processing while Mohs surgeons generally illustrated the Mohs layer map. Automated routine staining was used in most laboratory tests, and laboratory tests used similar staining techniques. Most respondents worked in private offices verses academic centers. Total staining time was significantly longer at academic medical centers versus private offices (7 vs 5 minutes, p = .01).
These findings provide an updated profile of current laboratory training and tissue preparation techniques at MMS practices across the country. Understanding the roles of the MH in laboratory functioning may help laboratories adopt best practices.
Mohs 组织病理技术员（MH）负责组织准备、切片和染色，这些是确保 Mohs 显微外科手术（MMS）成功的关键任务。
评估 MH 培训、实践环境以及特定组织学技术应用的当前规范。
使用 Survey Monkey 为美国 Mohs 组织病理技术学会的所有成员创建并分发了一份 16 个问题的调查问卷。
回复率为 30%。大多数 MH 是通过其他 MH 或 Mohs 外科医生接受在职培训的。Mohs 组织病理技术员主要负责组织处理相关的任务，而 Mohs 外科医生则主要负责描绘 Mohs 层图。大多数实验室检测都使用自动化常规染色，且实验室检测采用的染色技术相似。大多数受访者在私人诊所工作，而非学术中心。与私人诊所相比，学术医疗中心的总染色时间明显更长（7 分钟对 5 分钟，p =.01）。
这些发现提供了当前全国范围内 MMS 实践中实验室培训和组织准备技术的最新概况。了解 MH 在实验室功能中的角色可能有助于实验室采用最佳实践。
