乳糖阻遏蛋白头部与操纵基因的相互作用：核磁共振视角
The interaction of lac repressor headpiece with its operator: an NMR view.
作者信息
Boelens R, Lamerichs R M, Rullmann J A, van Boom J H, Kaptein R
机构信息
Department of Chemistry, University of Utrecht, The Netherlands.
出版信息
Protein Seq Data Anal. 1988;1(6):487-98.
Analysis of nuclear Overhauser enhancements in two-dimensional NMR spectra of the complex of lac repressor headpiece with a 14 base pair lac operator fragment shows that the second helix of the headpiece binds in the major groove of DNA, as has been suggested. The orientation of this helix is approximately 180 degrees different from the proposed models and from that found in the X-ray structure of the 434 repressor-operator complex. The model of the lac headpiece-operator complex provides a good explanation for a large amount of biochemical, genetic and physical data.
对乳糖阻遏蛋白头部片段与一个14碱基对的乳糖操纵基因片段形成的复合物的二维核磁共振谱中的核Overhauser效应进行分析表明，正如所推测的那样，头部片段的第二个螺旋结合在DNA的大沟中。该螺旋的方向与提出的模型以及在434阻遏蛋白 - 操纵基因复合物的X射线结构中发现的方向大约相差180度。乳糖阻遏蛋白头部 - 操纵基因复合物的模型为大量生化、遗传和物理数据提供了很好的解释。
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