Pang Yu-Zhou, Huang An, Zhang Qing-Huai, Chen Xiao-Yue, Fang Gang
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2016 Aug;36(8):981-985.
Objective To observe the effect of Longzuan Tongbi Recipe (LTR) on Fas/FasL sys- tems in serum and synovium of collagen-induced arthritis (CIA) rats. Methods Ten rats were randomly selected from 60 male Wistar rats as a normal control group. CIA model was prepared by injecting type II bovine collagen and incomplete Freund's adjuvant mixture in the rest 50 rats. After modeling rats were di- vided into the model group, the methotrexate (MTX) group, high, middle, and low dose LTR groups, 10 in each group. Normal saline was administered to rats in the model group by gastrogavage. MTX solution (0.27 mg/100 g) was administered to rats in the MTX group by gastrogavage, once per week for 4 succes- sive weeks. LTR (4.32, 2.16, 1.08 g/mL) was administered to rats in the 3 LTR groups by gastrogavage, twice per day for 30 successive days. Morphological changes of synovium were observed by HE staining. Expression levels of Fas/FasL in rat serum and synovium were quantitatively detected by ELISA. Results Normal synovium cells could be seen in the normal group. But they were obviously proliferated, fat cells in the lower synovium were reduced or deformed, fibroblasts were increased in the model group, accompa- nied with infiltration of lymphocytes and monocytes. All these changes were more obviously alleviated in the MTX group, and the 3 LTR groups. Compared withI the normal control group, Fas expression level in- creased in rat serum and synovium, serum FasL expression level decreased in the model group (P <0. 05, P <0. 01). Compared with the model group, Fas expression level decreased in rat serum and synovium in the MTX group, high and middle dose LTR groups; Fas expression level in rat serum increased in the MTX group and 3 LTR groups; Fas expression level in synovium increased in the MTX group, high and middle dose LTR groups (P <0. 05, P <0. 01). Compared with the MTX group, Fas expression level in serum of the low dose LTR group, and Fas expression level in synovium of low and middle dose LTR groups was elevat- ed; Fas expression level in serum and synovium of the high dose LTR group was reduced; FasL expres- sion level in serum and synovium of low and middle dose LTR groups was reduced; FasL expression level in serum and synovium increased of the high dose LTR group (P <0. 05, P <0. 01). Conclusion LTR could control and treat rheumatoid arthritis, and its mechanism might lie in regulating. Fas/FasL systems media- ted cell apoptosis, and relieving pathological reaction of rheumatoid arthritis.
目的 观察龙钻通痹方（LTR）对胶原诱导性关节炎（CIA）大鼠血清及滑膜中Fas/FasL系统的影响。方法 从60只雄性Wistar大鼠中随机选取10只作为正常对照组。将其余50只大鼠注射Ⅱ型牛胶原和不完全弗氏佐剂混合物制备CIA模型。造模后将大鼠分为模型组、甲氨蝶呤（MTX）组、LTR高、中、低剂量组，每组10只。模型组大鼠灌胃给予生理盐水。MTX组大鼠灌胃给予MTX溶液（0.27 mg/100 g），每周1次，连续4周。3个LTR组大鼠分别灌胃给予LTR（4.32、2.16、1.08 g/mL），每日2次，连续30天。通过HE染色观察滑膜的形态学变化。采用ELISA法定量检测大鼠血清及滑膜中Fas/FasL的表达水平。结果 正常组可见正常滑膜细胞。模型组滑膜细胞明显增殖，滑膜下层脂肪细胞减少或变形，成纤维细胞增多，伴有淋巴细胞和单核细胞浸润。MTX组及3个LTR组上述变化均明显减轻。与正常对照组比较，模型组大鼠血清及滑膜中Fas表达水平升高，血清FasL表达水平降低（P<0.05，P<0.01）。与模型组比较，MTX组、LTR高、中剂量组大鼠血清及滑膜中Fas表达水平降低；MTX组及3个LTR组大鼠血清中Fas表达水平升高；MTX组、LTR高、中剂量组大鼠滑膜中Fas表达水平升高（P<0.05，P<0.01）。与MTX组比较，LTR低剂量组血清中Fas表达水平及LTR低、中剂量组滑膜中Fas表达水平升高；LTR高剂量组血清及滑膜中Fas表达水平降低；LTR低、中剂量组血清及滑膜中FasL表达水平降低；LTR高剂量组血清及滑膜中FasL表达水平升高（P<0.05，P<0.01）。结论 LTR可控制和治疗类风湿关节炎，其机制可能在于调节Fas/FasL系统介导的细胞凋亡，减轻类风湿关节炎的病理反应。
