National Key Research Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, PR China; Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China.
J Ethnopharmacol. 2019 Apr 24;234:36-43. doi: 10.1016/j.jep.2019.01.008. Epub 2019 Jan 11.
Shenfu injection is a popular Chinese herbal formula that has been widely used in the treatment of shock in China.
To investigate the effect of Shenfu injection on lipopolysaccharide (LPS)-induced septic shock in rabbits.
We established a septic shock model in rabbits by administering an intravenous injection of 0.6 mg/kg LPS to anesthetized rabbit, and 15 min after LPS challenge, the rabbits were intravenously administered the Shenfu injection. In these in vivo experiments, the jugular vein of the rabbits was cannulated for LPS and drug administration, and the right common carotid artery was cannulated to record the mean arterial pressure (MAP) over a 6-h period. In addition, various serum biochemical parameters, including lactate dehydrogenase (LDH), aspartate aminotransferase (AST), glutamate transaminase (ALT), creatinine (Cre), and urea nitrogen (Urea), were measured at 0, 3, and 6 h. Serum LPS levels at 6 h were determined by the test kit. And histological changes in the heart, liver and kidney tissues were observed by HE staining. Furthermore, some related small molecules in the heart tissues were detected by MALDI-TOF-MSI.
We found that Shenfu injection can increase the MAP, decrease the serum LPS, LDH and AST levels, and improve the tissue morphology of the heart, liver and kidney in rabbits with LPS-induced septic shock. In addition, Shenfu injection can increase the contents of ATP and taurine while reducing the content of AMP in the heart tissue during septic shock.
These results indicate that Shenfu injection exerts a protective effect on LPS-induced septic shock in rabbits.
参附注射液是一种广受欢迎的中草药配方，已在中国广泛用于治疗休克。
研究参附注射液对脂多糖（LPS）诱导的兔脓毒性休克的作用。
通过向麻醉兔静脉注射 0.6mg/kg LPS 建立脓毒性休克模型，在 LPS 攻击后 15min，兔静脉注射参附注射液。在这些体内实验中，兔颈静脉用于 LPS 和药物给药，右颈总动脉用于记录 6 小时内的平均动脉压（MAP）。此外，在 0、3 和 6 小时测量各种血清生化参数，包括乳酸脱氢酶（LDH）、天门冬氨酸氨基转移酶（AST）、谷氨酸转氨酶（ALT）、肌酐（Cre）和尿素氮（Urea）。通过试剂盒测定 6 小时时的血清 LPS 水平。通过 HE 染色观察心脏、肝脏和肾脏组织的组织学变化。此外，通过 MALDI-TOF-MSI 检测心脏组织中的一些相关小分子。
我们发现参附注射液可增加 MAP，降低 LPS、LDH 和 AST 血清水平，改善 LPS 诱导的兔脓毒性休克时心脏、肝脏和肾脏的组织形态。此外，参附注射液可增加脓毒性休克时心脏组织中 ATP 和牛磺酸的含量，同时降低 AMP 的含量。
这些结果表明参附注射液对 LPS 诱导的兔脓毒性休克具有保护作用。
