内皮型一氧化氮合酶基因 G894T 多态性与血氧运输的关系。
Association of endothelial nitric oxide synthase gene G894T polymorphism with blood oxygen transport.
机构信息
Department of Physiology, Grodno State Medical University, 230009, Gorky str. 80, Grodno, Belarus.
出版信息
Nitric Oxide. 2019 Mar 1;84:45-49. doi: 10.1016/j.niox.2019.01.007. Epub 2019 Jan 11.
BACKGROUND
Nitric oxide (NO) is involved in formation of oxygen transport function of blood and oxygen transport in tissues by means of interaction with haemoglobin. Endothelial nitric oxide synthase gene G894T polymorphism affects the expression and the activity of the NO synthase enzyme. The influence of this polymorphism on the formation of oxygen-dependent processes in the body has not been completely investigated.
OBJECTIVE
To evaluate the associations between G894T polymorphism of the endothelial nitric oxide synthase gene and the condition of oxygen transport function of blood in healthy male volunteers.
METHODS
The study subjects were healthy young males aged 18-24 years (n = 165). The blood was drawn from the cubital vein at rest after 12 h following the last food intake. G984T polymorphism and the blood oxygen indices pO, CvO, SO, pH, р50 (the temperature was 37°С, рН = 7.4, рСО = 40 mm Hg) and р50 (actual temperature, рН and рСО), etc. were determined.
RESULTS
In persons with the TT-genotype, the oxygen content in venous blood was 48.7% (q = 0.019) lower compared to subjects with the GT-genotype and 49.4% (q = 0.019) lower compared to subjects with the GG-genotype. The saturation of blood in carriers of the TT-genotype was 32.4% (q = 0.021) and 35.9% (q = 0.019) lower as opposed to subjects with the GT-genotype and the GG-genotype, respectively. In blood of subjects with the TT-genotype, the oxygen tension was 26.1% (q = 0.019) lower as compared to subjects with the GT-genotype and 27.4% (q = 0.019) lower as opposed to the GG-genotype. In turn, volunteers having a common allele in their genotype (GG + GT) demonstrated oxygen tension to be 26.7% (q = 0.019) higher compared to subjects with the TT-genotype. The blood pH values of the subjects having the recessive genotype were 0.022 units lower compared to the GG (q = 0.044) and GT (q = 0.042) genotypes. In volunteers with the TT-genotype, the p50 parameter was 5.8% (q = 0.027) lower compared to subjects with the GT-genotype and 6.8% (q = 0.019) lower compared to volunteers with the GG-genotype. In persons with two T-alleles in their genotype, p50 was 5.4% (q = 0.019) lower compared to subjects with the GT-genotype and 6.4% (q = 0.019) lower compared to persons with the GG-genotype.
CONCLUSION
The T-allele of G894T polymorphism is associated with low values for oxygen content, oxygen tension, acidic pH shift and increased haemoglobin affinity for oxygen under standard and real conditions. The presence of a minor allele in G894T polymorphism of the NOS3 gene contributes to formation of oxygen transport function of blood.
背景
一氧化氮（NO）通过与血红蛋白相互作用，参与血液的氧气运输功能和组织中的氧气运输。内皮型一氧化氮合酶基因 G894T 多态性影响一氧化氮合酶的表达和活性。这种多态性对体内氧依赖过程的形成的影响尚未完全研究。
目的
评估内皮型一氧化氮合酶基因 G894T 多态性与健康男性志愿者血液氧气运输功能状况之间的关系。
方法
研究对象为 18-24 岁的健康年轻男性（n=165）。在最后一次进食后 12 小时，从肘部静脉抽取休息时的血液。测定 G984T 多态性和血液氧指数 pO、CvO、SO、pH、р50（温度为 37°C、рН=7.4、рСО=40mmHg）和 р50（实际温度、рН和 рСО）等。
结果
在 TT 基因型的个体中，静脉血中的氧含量比 GT 基因型个体低 48.7%（q=0.019），比 GG 基因型个体低 49.4%（q=0.019）。TT 基因型携带者的血液饱和度比 GT 基因型个体低 32.4%（q=0.021）和 35.9%（q=0.019），比 GG 基因型个体低 35.9%（q=0.019）。TT 基因型个体的血氧分压比 GT 基因型个体低 26.1%（q=0.019），比 GG 基因型个体低 27.4%（q=0.019）。相反，在其基因型中具有共同等位基因的志愿者（GG+GT）的血氧分压比 TT 基因型个体高 26.7%（q=0.019）。具有隐性基因型的受试者的血液 pH 值比 GG（q=0.044）和 GT（q=0.042）基因型低 0.022 个单位。在 TT 基因型的志愿者中，p50 参数比 GT 基因型个体低 5.8%（q=0.027），比 GG 基因型个体低 6.8%（q=0.019）。在基因型中存在两个 T 等位基因的个体中，p50 比 GT 基因型个体低 5.4%（q=0.019），比 GG 基因型个体低 6.4%（q=0.019）。
结论
G894T 多态性的 T 等位基因与标准和实际条件下氧含量、氧分压、酸性 pH 值变化和血红蛋白对氧亲和力增加的低值相关。NOS3 基因 G894T 多态性中的小等位基因的存在有助于血液氧气运输功能的形成。
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