Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, China.
Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, China.
Bioresour Technol. 2019 Mar;276:343-348. doi: 10.1016/j.biortech.2019.01.008. Epub 2019 Jan 4.
In this study, the fermentable sugar released from cotton stalk (CS), which were pretreated by instant catapult steam explosion (SE) combined with different concentrations of strong monobasic acid (HCl), weak monobasic acid (CHCOOH), strong monobasic alkali (NaOH) and weak monobasic alkali (NH·HO), followed by hydrolysis in cellulase/xylanase mixed enzyme solutions, were comparably investigated. The highest yield of 73.22% of fermentable sugar yield was obtained in SE-2.4 MPa-5%NH·HO treated CS substrates, which was 5.14 times higher than that from enzymatic hydrolysis (EH) of raw CS. Furthermore, evaluation of monolignins content (H, G, S) in different CS samples suggested that substrates rich in guaiacyl (G) and syringyl (S) would generate a higher efficiency of enzymatic saccharification. Therefore, the slight genetic modification of monolignins for cotton stalk might be a potential way to enhance biomass degradation and transformation.
在这项研究中，对经过瞬时弹射蒸汽爆炸（SE）预处理的棉秆（CS）中释放的可发酵糖进行了研究，预处理时采用了不同浓度的强酸（HCl）、弱酸（CHCOOH）、强碱（NaOH）和弱碱（NH·HO），然后在纤维素酶/木聚糖酶混合酶溶液中进行水解。比较了不同预处理条件下可发酵糖得率。在 SE-2.4 MPa-5%NH·HO 处理的 CS 底物中，可发酵糖的最高收率为 73.22%，比原始 CS 的酶解（EH）高 5.14 倍。此外，对不同 CS 样品中单宁含量（H、G、S）的评估表明，富含愈创木基（G）和丁香基（S）的底物将产生更高的酶解效率。因此，对棉秆中单宁进行轻微的遗传修饰可能是提高生物质降解和转化效率的一种潜在途径。
