Hussain Misbah, Abbas Shahid, Bilal Ahmed, Aslam Muhammad Naeem, Awan Fazli Rabbi
Diabetes and Cardio-Metabolic Disorders Lab, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
Pakistan Institute of Engineering and Applied Sciences (PIEAS), Nilore, Islamabad, Pakistan.
Exp Clin Endocrinol Diabetes. 2020 Feb;128(2):82-88. doi: 10.1055/a-0824-6515. Epub 2019 Jan 14.
Angiotensin converting enzyme (ACE), as part of renin angiotensin aldosterone system, is involved in blood pressure regulation and control several physiological functions. Insertion/Deletion (I/D) polymorphism of has pronounced effects on development of metabolic diseases like diabetes, cardiovascular diseases (CVDs) and hypertension. However, association of I/D polymorphism with risk of diabetes in CVD patients is not known. The aim of present study was to check the association of I/D polymorphism with risk of diabetes in subjects with CVD. For this, 531 subjects were sampled and divided into 3 groups; G1-H: (healthy controls, n=117), G2-CN: (cardiac patients without diabetes, n=271) and G3-CD: (cardiac patients with diabetes, n=143). Genotyping of I/D polymorphism was done by polymerase chain reaction. Allelic and genotypic frequencies were in Hardy Weinberg Equilibrium (χ2=0.11, p>0.05) and revealed high prevalence of I allele (55%) among all groups. However, II genotype was more common (37%) in G3-CD: group. Level of glucose was also higher in subjects with II genotype than DD genotype (12.6±6.3 mmol/L vs. 9.7±5.1 mmol/L). Logistic regression analysis revealed that II genotype increase the risk of diabetes in CVD patients by ~2 times [OR=1.94, CI: 1.24-3.01, p=0.03]; however, this association did not reach the significance level when adjusted for age and gender. In conclusion, I/D polymorphism influence the risk of diabetes in CVD patients and II increases this risk by ~2 fold.
血管紧张素转换酶（ACE）作为肾素-血管紧张素-醛固酮系统的一部分，参与血压调节并控制多种生理功能。其插入/缺失（I/D）多态性对糖尿病、心血管疾病（CVD）和高血压等代谢性疾病的发生发展有显著影响。然而，I/D多态性与CVD患者患糖尿病风险之间的关联尚不清楚。本研究的目的是检测I/D多态性与CVD患者患糖尿病风险之间的关联。为此，选取了531名受试者并分为3组；G1-H：（健康对照组，n = 117），G2-CN：（无糖尿病的心脏病患者，n = 271）和G3-CD：（有糖尿病的心脏病患者，n = 143）。通过聚合酶链反应对I/D多态性进行基因分型。等位基因和基因型频率符合哈迪-温伯格平衡（χ2 = 0.11，p>0.05），且显示所有组中I等位基因的患病率都很高（55%）。然而，II基因型在G3-CD组中更为常见（37%）。II基因型受试者的血糖水平也高于DD基因型受试者（12.6±6.3 mmol/L对9.7±5.1 mmol/L）。逻辑回归分析显示，II基因型使CVD患者患糖尿病的风险增加约2倍[OR = 1.94，CI：1.24 - 3.01，p = 0.03]；然而，在调整年龄和性别后，这种关联未达到显著水平。总之，I/D多态性影响CVD患者患糖尿病的风险，II基因型使该风险增加约2倍。
