Chermprapai Suttiwee, Ederveen Thomas H A, Broere Femke, Broens Els M, Schlotter Yvette M, van Schalkwijk Saskia, Boekhorst Jos, van Hijum Sacha A F T, Rutten Victor P M G
Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, 3584 CL, the Netherlands; Department of Companion Animals Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand; Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Utrecht, 3584 CM, the Netherlands.
Centre for Molecular and Biomolecular Informatics (CMBI), Radboud University Nijmegen Medical Centre, Nijmegen, 6525 GA, the Netherlands; NIZO, Ede, 6718 ZB, the Netherlands.
Vet Microbiol. 2019 Feb;229:90-99. doi: 10.1016/j.vetmic.2018.12.022. Epub 2018 Dec 21.
Canine atopic dermatitis is a genetically predisposed inflammatory and pruritic allergic skin disease that is often complicated by (secondary) bacterial and fungal (yeast) infections. High-throughput DNA sequencing was used to characterize the composition of the microbiome (bacteria and fungi) inhabiting specific sites of skin in healthy dogs and dogs with atopic dermatitis (AD) before and after topical antimicrobial treatment. Skin microbiome samples were collected from six healthy control dogs and three dogs spontaneously affected by AD by swabbing at (non-) predilection sites before, during and after treatment. Bacteria and fungi were profiled by Illumina sequencing of the 16S ribosomal RNA gene of bacteria (16S) and the internally transcribed spacer of the ribosomal gene cassette in fungi (ITS). The total cohort of dogs showed a high diversity of microbes on skin with a strong individual variability of both 16S and ITS profiles. The genera of Staphylococcus and Porphyromonas were dominantly present both on atopic and healthy skin and across all skin sites studied. In addition, bacterial and fungal alpha diversity were similar at the different skin sites. The topical antimicrobial treatment increased the diversity of bacterial and fungal compositions in course of time on both AD and healthy skin.
犬特应性皮炎是一种具有遗传易感性的炎症性瘙痒性过敏性皮肤病，常并发（继发性）细菌和真菌（酵母）感染。采用高通量DNA测序技术，对健康犬和患有特应性皮炎（AD）的犬在局部抗菌治疗前后，皮肤特定部位微生物群（细菌和真菌）的组成进行表征。通过在治疗前、治疗期间和治疗后，在（非）好发部位擦拭取样，从6只健康对照犬和3只自发患AD的犬身上采集皮肤微生物群样本。通过对细菌16S核糖体RNA基因（16S）和真菌核糖体基因盒的内转录间隔区（ITS）进行Illumina测序，对细菌和真菌进行分析。整个犬群显示皮肤微生物种类高度多样，16S和ITS图谱均具有很强的个体变异性。葡萄球菌属和卟啉单胞菌属在特应性皮肤和健康皮肤上以及所有研究的皮肤部位均占主导地位。此外，不同皮肤部位的细菌和真菌α多样性相似。局部抗菌治疗随着时间的推移增加了AD皮肤和健康皮肤中细菌和真菌组成的多样性。
