Laboratory for Stem Cells Manipulation and Cryopreservation, Dpt of Transfusion Medicine, ASST Spedali Civili, Brescia, Italy.
Chair of Hematology, Unit of Blood Diseases and Stem Cell Transplantation, University of Brescia, ASST Spedali Civili, Brescia, Italy.
Sci Rep. 2019 Jan 14;9(1):87. doi: 10.1038/s41598-018-36442-9.
Physio-pathologic interrelationships between endothelial layer and graft-versus-host disease (GVHD) have been described leading to assess the entity "endothelial GVHD" as the early step for clinical manifestations of acute GVHD. The availability of the CellSearch system has allowed us to monitor Circulating Endothelial Cells (CEC) changes in allogeneic hematopoietic stem cell transplantation (allo-HSCT) as useful tool to help clinicians in GVHD diagnostic definition. We have compared CEC counts generated by an ad hoc designed polychromatic-flowcytometry (PFC) Lyotube with those of the CellSearch system. CEC were counted in parallel at 5 timepoints in 50 patients with malignant hematologic disorders undergoing allo-HSCT (ClinicalTrials.gov, NCT02064972). Spearman rank correlation showed significant association between CEC values at all time points (p = 0.0001). The limits of agreement was demonstrated by Bland Altman plot analysis, showing bias not significant at T1, T3, T4, while at T2 and T5 resulted not estimable. Moreover, Passing Bablok regression analysis showed not significant differences between BD Lyotube and CellSearch system. We show that CEC counts, generated with either the CellSearch system or the PFC-based panel, have a superimposable kinetic in allo-HSCT patients and that both counting procedures hold the potential to enter clinical routine as a suitable tool to assist clinicians in GVHD diagnosis.
内皮细胞层与移植物抗宿主病（GVHD）之间的生理病理相互关系已被描述，这导致评估实体“内皮 GVHD”作为急性 GVHD 临床表现的早期步骤。CellSearch 系统的可用性使我们能够监测同种异体造血干细胞移植（allo-HSCT）中循环内皮细胞（CEC）的变化，作为帮助临床医生进行 GVHD 诊断定义的有用工具。我们比较了通过专门设计的多色流式细胞术（PFC） Lyotube 生成的 CEC 计数与 CellSearch 系统的计数。在 50 名患有恶性血液系统疾病并接受 allo-HSCT 的患者中，在 5 个时间点同时进行 CEC 计数（ClinicalTrials.gov，NCT02064972）。Spearman 秩相关显示所有时间点的 CEC 值均存在显著相关性（p=0.0001）。 Bland Altman 图分析显示一致性界限，T1、T3 和 T4 时偏倚不显著，而 T2 和 T5 时偏倚不可估计。此外，Passing Bablok 回归分析显示 BD Lyotube 和 CellSearch 系统之间无显著差异。我们表明，使用 CellSearch 系统或基于 PFC 的面板生成的 CEC 计数在 allo-HSCT 患者中具有可叠加的动力学，并且两种计数程序都有可能作为一种辅助临床医生进行 GVHD 诊断的合适工具进入临床常规。
