DNA条形码揭示了印度东北部濒危淡水龟身上舌蛭科物种的关联。
DNA Barcoding Reveals Association of Glossiphoniidae Species on Endangered Freshwater Turtles in Northeast India.
作者信息
Kundu Shantanu, Kumar Vikas, Tyagi Kaomud, Pakrashi Avas, Laskar Boni Amin, Chandra Kailash
机构信息
Centre for DNA Taxonomy, Molecular Systematics Division, Zoological Survey of India, Kolkata, 700053, India.
Freshwater Biology Regional Centre, Zoological Survey of India, Hyderabad, 500048, India.
出版信息
Acta Parasitol. 2019 Mar;64(1):213-217. doi: 10.2478/s11686-018-00023-7. Epub 2019 Jan 14.
BACKGROUND
The identification of Glossiphoniidae species is often confusing due to the lack of both morphological and molecular data worldwide.
PURPOSE
The study aimed to identify the collected leech specimens from two endangered freshwater turtles, Chitra indica and Pangshura sylhetensis from northeast India.
METHODS
We generated DNA barcode sequences and estimated the genetic distances and phylogenetic relationship of the studied taxa with another 14 Glossiphoniidae genera (114 barcode sequences).
RESULTS
The high genetic distinctiveness (17.9 to 26.3%) and distinct clustering in Neighbor-Joining (NJ) phylogeny, we identified the studied specimens as Glossiphoniidae sp. We assumed that the studied specimens might be a new member of Glossiphoniidae or an extant species without DNA information.
CONCLUSION
Hence, the study recommends further sampling of leeches from the similar host as well as from same and various localities and also generates the molecular data to perceive the exact diversity. The aimed study is also helpful to encourage the awareness and conservation management of freshwater turtles and other threatened animals.
背景
由于全球范围内形态学和分子数据的缺乏，舌蛭科物种的鉴定常常令人困惑。
目的
本研究旨在鉴定从印度东北部两种濒危淡水龟，即印度锯背龟和锡尔赫特棱背龟采集的水蛭标本。
方法
我们生成了DNA条形码序列，并估计了所研究分类群与其他14个舌蛭科属（114个条形码序列）的遗传距离和系统发育关系。
结果
基于较高的遗传独特性（17.9%至26.3%）以及在邻接法（NJ）系统发育中的明显聚类，我们将所研究的标本鉴定为舌蛭科物种。我们推测所研究的标本可能是舌蛭科的一个新成员，或者是一个没有DNA信息的现存物种。
结论
因此，该研究建议进一步从相似宿主以及相同和不同地点采集水蛭样本，并生成分子数据以了解确切的多样性。该目标研究也有助于提高对淡水龟和其他濒危动物的认识及保护管理。
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