酿酒酵母中染色体稳定性的遗传控制。
Genetic control of chromosome stability in the yeast Saccharomyces cerevisiae.
作者信息
Pashina O B, Nikolaishwili N T, Tsouladze A M, Larionov V L
机构信息
Institute of Cytology, Academy of Sciences of the USSR, Leningrad.
出版信息
Yeast. 1988 Dec;4(4):257-69. doi: 10.1002/yea.320040404.
We have identified four new genetic loci: CHL2 (on chromosome XII), CHL3 (on chromosome XII); CHL4 (on chromosome IV), and CHL5 (on chromosome IX), controlling mitotic transmission of yeast chromosomes. The frequency of loss of chromosomes is 10-100-fold higher in chl5, chl2, chl3 and chl4 mutants than observed in wild-type strains. The mutants also show unstable maintenance of artificial circular minichromosomes with various chromosomal replicators (ARS) and one of the centromeric loci (CEN3, CEN4, CEN5 or CEN6). The instability of minichromosomes in the chl5, chl2, and chl4 mutants is due to the loss of minichromosomes in mitosis (1:0 segregation). In the chl3 mutant the instability of artificial minichromosomes is due to nondisjunction (2:0 segregation). The CHL3 gene therefore appears to affect the segregation of chromosomes during cell division.
我们已经鉴定出四个新的基因位点
CHL2（位于第十二号染色体上）、CHL3（位于第十二号染色体上）、CHL4（位于第四号染色体上）和CHL5（位于第九号染色体上），它们控制着酵母染色体的有丝分裂传递。chl5、chl2、chl3和chl4突变体中染色体丢失的频率比野生型菌株中观察到的高10至100倍。这些突变体还表现出带有各种染色体复制起点（ARS）和一个着丝粒位点（CEN3、CEN4、CEN5或CEN6）的人工环状微型染色体的维持不稳定。chl5、chl2和chl4突变体中微型染色体的不稳定性是由于有丝分裂过程中微型染色体的丢失（1:0分离）。在chl3突变体中，人工微型染色体的不稳定性是由于不分离（2:0分离）。因此，CHL3基因似乎影响细胞分裂过程中染色体的分离。
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