Zhou Kun, Dong Jinyi, Zhou Yihao, Dong Jinchen, Wang Meng, Wang Qiangbin
CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine and i-Lab, CAS Center for Excellence in Brain Science, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, 30322, USA.
Small. 2019 Jun;15(26):e1804044. doi: 10.1002/smll.201804044. Epub 2019 Jan 15.
Nucleic acids and proteins are the two primary building materials of living organisms. Over the past decade, artificial DNA-protein hybrid structures have been pursued for a wide range of applications. DNA nanotechnology, in particular, has dramatically expanded nanoscale molecule engineering and contributed to the spatial arrangement of protein components. Strategies for designing site-specific coupling of DNA oligomers to proteins are needed in order to allow for precise control over stoichiometry and position. Efforts have also been focused on coassembly of protein-DNA complexes by engineering their fundamental molecular recognition interactions. This Concept focuses on the precise manipulation of DNA-protein nanoarchitectures. Particular attention is paid to site-selectivity within DNA-protein conjugates, regulation of protein orientation using DNA scaffolds, and coassembly principles upon unique structural motifs. Current challenges and future directions are also discussed in the design and application of DNA-protein nanoarchitectures.
核酸和蛋白质是生物体的两种主要构建材料。在过去十年中，人们一直在探索人工DNA-蛋白质杂交结构，以用于广泛的应用。特别是DNA纳米技术，极大地扩展了纳米级分子工程，并有助于蛋白质组件的空间排列。为了能够精确控制化学计量和位置，需要设计将DNA寡聚物与蛋白质进行位点特异性偶联的策略。人们还致力于通过改造蛋白质-DNA复合物的基本分子识别相互作用来实现它们的共组装。本概念聚焦于DNA-蛋白质纳米结构的精确操纵。特别关注DNA-蛋白质缀合物中的位点选择性、使用DNA支架调节蛋白质方向以及基于独特结构基序的共组装原理。还讨论了DNA-蛋白质纳米结构设计与应用中的当前挑战和未来方向。
