Lange Máximo E, Uwiera Richard R E, Inglis G Douglas
Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, Alberta, Canada.
Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
Curr Protoc Mouse Biol. 2019 Mar;9(1):e59. doi: 10.1002/cpmo.59. Epub 2019 Jan 15.
Gnotobiotic mice are an established, robust model utilized in current research to study host-microbiota interactions. For years isolators have been used to rear germ-free and gnotobiotic mice. However, isolators can be costly and the segregation of treatments within the same isolator is problematic. Recently, methodologies for housing germ-free mice in specially designed individually ventilated cages (IVCs) operated under barrier mode have been developed; however, this equipment is costly and its operation in barrier mode for research involving germ-free mice and pathogens is not permissible under modern biosafety and biosecurity regulations. This article describes a method to house germ-free mice in a commonly available conventional IVC system operated under containment mode. This technique allows researchers to maintain the germ-free or gnotobiotic status of mice tested up to 4 weeks with weekly handling while working with pathogens using each IVC as a separate experimental unit. © 2019 Her Majesty the Queen in Right of Canada.
悉生小鼠是当前研究中用于研究宿主-微生物群相互作用的一种成熟、可靠的模型。多年来，隔离器一直用于饲养无菌和悉生小鼠。然而，隔离器成本高昂，且在同一隔离器内进行不同处理的分隔存在问题。最近，已经开发出在屏障模式下运行的专门设计的独立通风笼（IVC）中饲养无菌小鼠的方法；然而，这种设备成本高昂，并且根据现代生物安全和生物安保法规，在涉及无菌小鼠和病原体的研究中以屏障模式运行是不允许的。本文描述了一种在遏制模式下运行的常用传统IVC系统中饲养无菌小鼠的方法。该技术使研究人员能够在每周处理的情况下，将受试小鼠的无菌或悉生状态维持长达4周，同时将每个IVC作为一个单独的实验单元与病原体一起使用。© 2019加拿大女王陛下。
