Haouas Mouna, Chebbi Brahim, Golub Ilya
J Opt Soc Am A Opt Image Sci Vis. 2019 Jan 1;36(1):124-131. doi: 10.1364/JOSAA.36.000124.
Light sheet illumination is the basis in developing light sheet microscopy (LSM), a technique with significant advantages compared with other classical techniques. Most proposed optical systems to generate light sheets for LSM use many optical elements, which require extensive adjustments and are costly; moreover, they generate a nonuniform or semiuniform light sheet or have a short depth of field (DOF). A simple scheme using a pair of double slits and a cylindrical lens for generating a quasi-nondiffracting 2D light sheet was reported in Opt. Lett.40, 5121 (2015)OPLEDP0146-959210.1364/OL.40.005121. In the present investigation, we elaborate on the optimization of the mask used. As the separation between the two slits increases, the light sheet becomes thinner and the DOF smaller and vice versa. The slits' width does not affect the light sheet thickness, but it does affect the intensity of the side lobes. For convergence angles of the inner slits from 0.75° to 8°, an optimum ratio of the slits' separation/width of 2.182 is recommended. The obtained light sheet is quasi-diffraction-free, namely, while its DOF is comparable with that of a Gaussian beam, its diffraction broadening is substantially smaller. We also add to the previously developed configuration a Powell lens in order to expand the beam in the spanwise direction while keeping nearly constant intensity in this dimension. We perform scalar diffraction theory calculations and conduct measurements showing the nearly constant intensity in the significantly broadened span of the light sheet. Potential applications for the augmented width include imaging of certain large embryos, laser micromachining, and microparticle image velocimetry.
光片照明显微技术(LSM)的发展是以光片照明为基础的,与其他传统技术相比,该技术具有显著优势。大多数为LSM生成光片而提出的光学系统使用了许多光学元件,这需要大量的调整且成本高昂;此外,它们生成的是不均匀或半均匀的光片,或者景深(DOF)较短。《光学快报》40, 5121 (2015)OPLEDP0146 - 959210.1364/OL.40.005121报道了一种使用一对双缝和一个柱面透镜生成准无衍射二维光片的简单方案。在本研究中,我们详细阐述了所用掩模的优化。随着两条狭缝之间的间距增加,光片会变薄,景深变小,反之亦然。狭缝的宽度不影响光片厚度,但会影响旁瓣强度。对于内狭缝的会聚角从0.75°到8°,建议狭缝间距与宽度的最佳比值为2.182。所获得的光片是准无衍射的,即,虽然其景深与高斯光束相当,但其衍射展宽要小得多。我们还在先前开发的配置中添加了一个鲍威尔透镜,以便在展向方向上扩展光束,同时在该维度上保持强度几乎恒定。我们进行了标量衍射理论计算并进行了测量,结果表明在光片显著展宽的范围内强度几乎恒定。光片展宽后的潜在应用包括对某些大型胚胎的成像、激光微加工和微粒图像测速。