Ma Jin, Chen Ting, Wang Yi Wei, Zhao Chan, Li Dong Hui, Wang Meng, Gan Lin Yang, Zhong Yong
Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, 430060 China.
Chin Med Sci J. 2018 Dec 30;33(4):252-259. doi: 10.24920/003477.
Objective To assess the in vivo dynamic blood flow features of posterior optic nerve head (ONH) in rat model of nonarteritic anterior ischemic optic neuropathy (rNAION). Methods rNAION was established with Rose Bengal and argon green laser in Sprague-Dawley rats. Fundus photography and fundus fluorescein angiography (FFA) were performed to assess the dynamic changes of optic disc in morphology in 90 days and in blood perfusion in 3 hours after the induction of disease. Histological examinations were performed to evaluate the success of modeling. The dynamic blood flow kinetics of posterior ONH in rNAION were measured by Laser Doppler Flowmetry (LDF) on the day 3, 7, 14, 21, and 40 after the disease induction. One-way ANOVA, Student's t-test and Bonferroni adjustment were used for multiple comparisons of kinetic measurements of blood flow. Results Optic disc edema and subsequent resolution associated with the development of optic disc pallor were observed in rNAION. FFA showed that the optic disc was hypofluorescence in the early phase and hyperfluorescence in the late phase. Histological studies suggested edema and loosened tissues of ONH, loss of retinal ganglion cells (RGCs), optic nerve substance and gliosis. Compared to the naive rats, the blood flow kinetics of posterior ONH in rNAION significant reduced at each time point after modeling (F=175.06, P<0.0001). The reductions were specifically remarkable in 14 days after the disease induction (All P<0.01). Continuous blood perfusion reduction was found in rNAION, with significant alteration in 14 days after disease induction. Our results provided important information for understanding the hemodynamic changes in rNAION.
目的 评估非动脉炎性前部缺血性视神经病变大鼠模型（rNAION）后视神经乳头（ONH）的体内动态血流特征。方法 采用孟加拉玫瑰红和氩绿激光在Sprague-Dawley大鼠中建立rNAION模型。在疾病诱导后90天进行眼底照相和眼底荧光血管造影（FFA），以评估视盘形态的动态变化，并在3小时内评估血流灌注情况。进行组织学检查以评估建模的成功与否。在疾病诱导后的第3、7、14、21和40天，通过激光多普勒血流仪（LDF）测量rNAION后视神经乳头的动态血流动力学。采用单因素方差分析、Student's t检验和Bonferroni校正对血流动力学测量进行多重比较。结果 在rNAION中观察到视盘水肿及随后与视盘苍白发展相关的消退。FFA显示视盘在早期为低荧光，晚期为高荧光。组织学研究提示视神经乳头水肿和组织疏松、视网膜神经节细胞（RGCs）丢失、视神经实质和胶质增生。与正常大鼠相比，rNAION后视神经乳头的血流动力学在建模后的每个时间点均显著降低（F=175.06，P<0.0001）。在疾病诱导后14天，这种降低尤为显著（所有P<0.01）。在rNAION中发现血流灌注持续减少，在疾病诱导后14天有显著变化。我们的结果为理解rNAION中的血流动力学变化提供了重要信息。
