Bhau B S, Wakhlu A K
Plant Tissue Culture Laboratory, Department of Botany, University of Jammu, Jammu I80 006, Jammu & Kashmir, India.
J Genet Eng Biotechnol. 2015 Dec;13(2):215-219. doi: 10.1016/j.jgeb.2015.07.003. Epub 2015 Aug 8.
Elephant tusk cactus (Lem.) Lem. is an important attractive ornamental cactus. The plant produces offshoots from tubercles very rarely, and the seedlings exhibit slow growth and susceptibility to damping off. Slow growth and high demand in the cactus industry lead to finding an alternate fast propagation method.
An innovative technique based on axillary bud proliferation has been developed for an ornamental cactus (Lem.) Lem. Four different explant types formed multiple shoots on Murashige and Skoog (MS) medium. Of the two cytokinins, 6-Benzylaminopurine (BAP) and Kinetin (KN), BAP proved to be more effective for multiple shoot induction and shoot growth from different explant types. Longitudinally cut stem explants, when cultured on MS medium supplemented with 6.6 μM BAP give maximum axillary shoot proliferation (12.4 shoots). Type of explant significantly influenced the micropropagation rate. Type of carbon source used in the medium imparted a profound effect on shoot growth and dry weight. The maximum dry weight gain of the shoot was observed with 9% sucrose.
Development of an efficient micropropagation protocol which can be used to produce more than 10,000 rooted plantlets in 150 days from a single longitudinally divided shoot explant.
象牙仙人掌（Lem.）Lem. 是一种极具吸引力的观赏仙人掌。该植物极少从瘤状突起处长出侧枝，且幼苗生长缓慢，易患猝倒病。仙人掌产业中生长缓慢且需求高，促使人们寻找替代的快速繁殖方法。
已开发出一种基于腋芽增殖的创新技术用于观赏仙人掌（Lem.）Lem.。四种不同的外植体类型在Murashige和Skoog（MS）培养基上形成了多个芽。在两种细胞分裂素6-苄基腺嘌呤（BAP）和激动素（KN）中，BAP被证明对不同外植体类型的多芽诱导和芽生长更有效。纵向切割的茎外植体在添加6.6 μM BAP的MS培养基上培养时，腋芽增殖最多（12.4个芽）。外植体类型显著影响微繁殖率。培养基中使用的碳源类型对芽的生长和干重有深远影响。在9%蔗糖条件下观察到芽的最大干重增加。
开发了一种高效的微繁殖方案，该方案可用于在150天内从单个纵向分割的茎外植体生产出10000多株生根苗。
