Homchampa P, Sarasombath S, Suvatte V, Vongskul M
Department of Clinical Immunology, Faculty of Associated Medical Sciences, Khon Kaen University, Thailand.
Asian Pac J Allergy Immunol. 1988 Dec;6(2):95-102.
Natural killer (NK) cell activity against K-562 target cells and HNK-1+ cell levels were serially determined in peripheral blood of 62 Thai children with dengue hemorrhagic fever/dengue shock syndrome aged 4-12 years and 59 age-matched normal controls. The studies were performed on febrile stage, 1st and 2nd day of subsidence of fever (shock stage), 3rd and 4th day of subsidence of fever (early convalescent stage) and once again on the late convalescent stage (approximately 14-18 days after subsidence of fever). The study revealed that during the course of disease the NK cell activity was not changed significantly from the normal controls. In contrast, the levels of HNK-1+ cells, which exhibited almost all NK and killer cell functional activities, were significantly decreased in the febrile and the shock stages and were normal in the early and late convalescent stages. The NK cell activity, on the per-cell basis, was significantly increased in the early disease stage when compared to that of the later period of the disease and of the normal controls. The study also revealed that patients with grade III of disease severity exhibited significantly more NK cell functional activities per cell than grade II on febrile stage and the first day of shock. These results suggest that natural killer cells were active in defense against dengue viral infection and might play some role in the pathogenesis of dengue hemorrhagic fever/dengue shock syndrome. Their functions might also determine the severity of the disease.
在62名4至12岁患有登革出血热/登革休克综合征的泰国儿童及59名年龄匹配的正常对照儿童的外周血中，连续测定了其针对K-562靶细胞的自然杀伤（NK）细胞活性以及HNK-1+细胞水平。研究在发热期、热退第1天和第2天（休克期）、热退第3天和第4天（早期恢复期）以及再次在恢复期后期（热退后约14 - 18天）进行。研究显示，在疾病过程中，NK细胞活性与正常对照相比无显著变化。相反，几乎展现所有NK和杀伤细胞功能活性的HNK-1+细胞水平在发热期和休克期显著降低，而在早期和恢复期后期则正常。基于单个细胞而言，疾病早期的NK细胞活性相较于疾病后期及正常对照显著增加。研究还显示，疾病严重程度为III级的患者在发热期和休克第1天，其单个细胞的NK细胞功能活性显著高于II级患者。这些结果表明，自然杀伤细胞在抵御登革病毒感染中发挥作用，可能在登革出血热/登革休克综合征的发病机制中扮演一定角色。它们的功能也可能决定疾病的严重程度。
