Plant tissue culture independent mediated transformation strategy for upland cotton ().

A new method of transgenic development called "" transformation method, where is used to infect the plantlets but the steps of regeneration of plants is totally avoided. In this study, we have reported a simple method for efficient transformation of diploid cotton cv LRK-516 Anjali using EHA-105 harbouring recombinant binary vector plasmid with gene. Four day old plantlets were used for transformation. A vertical cut was made at the junction of cotyledonary leaves, moderately bisecting the shoot tip and exposing meristem cells at apical meristem. This site was infected with inoculum. The transgenic events obtained were tested positive for the presence of gene with promoter gene. They are also tested negative for vector backbone integration and contamination in T0 events. With this method a transformation frequency of 6.89% was reported for the cv LRK-516.