Johnson T S, Katz R L, Pershouse M
Cytology Technology, Inc., Houston, TX 77054.
Anal Quant Cytol Histol. 1988 Dec;10(6):423-58.
Flow cytometric (FCM) methodology represents a powerful analytical tool for screening and detecting abnormal and malignant cells, for subclassifying malignancies beyond conventional morphologic type and grade and for transcending light microscopic features by providing more biologically meaningful information. This paper reviews the historical background leading to the development of the FCM methodology and instrumentation that is presently being used for cytopathologic (and histopathologic) diagnosis, tumor subclassification and identification of aggressive cancers. Emphasis is placed on human carcinomas, with reference to hematologic malignancies when appropriate. Examples of FCM DNA content and cytokinetic techniques are described for the various cytologic sampling methods. The advantages, limitations and future prospects for the application of flow cytometric techniques in cytopathology are discussed.
流式细胞术（FCM）方法是一种强大的分析工具，可用于筛选和检测异常及恶性细胞，对恶性肿瘤进行超越传统形态学类型和分级的亚分类，并通过提供更具生物学意义的信息来超越光学显微镜特征。本文回顾了导致FCM方法和仪器发展的历史背景，目前该方法和仪器正用于细胞病理学（和组织病理学）诊断、肿瘤亚分类以及侵袭性癌症的识别。重点是人类癌症，并在适当的时候参考血液系统恶性肿瘤。针对各种细胞学采样方法描述了FCM DNA含量和细胞动力学技术的实例。讨论了流式细胞术技术在细胞病理学中应用的优点、局限性和未来前景。
