克氏锥虫 13C 标记的 O-聚糖标准品用于质谱分析。
Trypanosoma cruzi 13C-labeled O-Glycan standards for mass spectrometry.
机构信息
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
出版信息
Glycobiology. 2019 Apr 1;29(4):280-284. doi: 10.1093/glycob/cwy111.
Abstract
Trypanosoma cruzi is a protozoan parasite that causes Chagas disease, a debilitating condition that affects over 10 million humans in the American continents. In addition to its traditional mode of human entry via the "kissing bug" in endemic areas, the infection can also be spread in non-endemic countries through blood transfusion, organ transplantation, eating food contaminated with the parasites, and from mother to fetus. Previous NMR-based studies established that the parasite expresses a variety of strain-specific and developmentally-regulated O-glycans that may contribute to virulence. In this report, we describe five synthetic O-glycan analytical standards and show their potential to enable a more facile analysis of native O-glycan isomers based on mass spectrometry.
摘要
克氏锥虫是一种原生动物寄生虫，可引起恰加斯病，该病在美洲大陆影响着超过 1000 万人。除了在流行地区通过“接吻虫”的传统方式进入人体之外，这种感染还可以通过输血、器官移植、食用被寄生虫污染的食物以及从母亲传染给胎儿在非流行国家传播。以前基于 NMR 的研究确定寄生虫表达多种菌株特异性和发育调节的 O-聚糖，这些聚糖可能有助于毒力。在本报告中，我们描述了五个合成 O-聚糖分析标准品，并展示了它们在基于质谱法更轻松地分析天然 O-聚糖异构体方面的潜力。
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