Kanazawa M, Ikeda J, Sato J, Natoya Y, Ito H, Komeda K, Kawazu S, Kanazawa Y
Third Department of Internal Medicine, Tokyo Medical College, Japan.
Diabetes Res Clin Pract. 1988 Sep 5;5(3):201-4. doi: 10.1016/s0168-8227(88)80089-5.
Secretion of insulin and glucagon from perfused BB rat pancreas was examined in rats (5 weeks old; n = 6) before and 2 weeks after (13 weeks old; n = 5) the onset of diabetes mellitus. The secretion of insulin and glucagon was modified by increasing concentrations of glucose in the perfusate from 2.8 mM to 16.5 mM. In 5-week-old BB rats, glucagon secretion was higher at the low glucose concentration (2.8 mM) and insulin secretion lower at the high glucose concentration (16.5 mM) than the corresponding values in age-matched control rats. In 13-week-old BB rats, insulin secretion was undetectable irrespective of glucose concentration. Glucagon secretion was only moderately suppressed by the perfusion of high glucose (16.5 mM) and was significantly higher than that of age-matched controls. The reduction in insulin secretion observed in 5-week-old rats without insulitis indicated that pancreatic B cells of BB rats of this age are somehow injured by humoral factors rather than cell infiltration. Cell function was apparently modified indirectly by the destruction of neighboring B cells.
在糖尿病发病前（5周龄；n = 6）和发病2周后（13周龄；n = 5），对灌注的BB大鼠胰腺中胰岛素和胰高血糖素的分泌进行了检测。通过将灌注液中葡萄糖浓度从2.8 mM增加到16.5 mM来改变胰岛素和胰高血糖素的分泌。在5周龄的BB大鼠中，低葡萄糖浓度（2.8 mM）时胰高血糖素分泌较高，高葡萄糖浓度（16.5 mM）时胰岛素分泌低于年龄匹配的对照大鼠的相应值。在13周龄的BB大鼠中，无论葡萄糖浓度如何，均未检测到胰岛素分泌。高葡萄糖（16.5 mM）灌注仅适度抑制了胰高血糖素分泌，且其分泌显著高于年龄匹配的对照大鼠。在无胰岛炎的5周龄大鼠中观察到的胰岛素分泌减少表明，该年龄的BB大鼠胰腺β细胞受到体液因子而非细胞浸润的某种损伤。细胞功能显然是通过相邻β细胞的破坏而间接改变的。
