Xin Ming-Wei, Zhang Qiu-Xia, Zhao Hui, Yang Yi-Iong, He Jun-Qin, Zhang Ying, Liang Xin-Yun, Wu Ying, Yang Wei, Yin Xiao-Dan
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2016 Dec;36(12):1491-1495.
Objective To explore the effect of Wenshen Yangxue Recipe (WYR) on inhibin-ac- tivin-follistatin (INH-ACT-FS) system and gonadal hormone level in anovulatory rats. Methods Anovula- tory rat model was established in 76 rats (9 days old) by subcutaneous injecting testosterone propionate (1. 25 mg/0. 05 mL for each rat) from the nape. Totally 58 successfully modeled rats were divided into 5 groups according to random digit table, i.e., the model group (n =10), the Western medicine (WM) group (n =12), high, middle, and low dose WYR groups (n =12). Besides, another ten 22-day old rats were recruited as a normal group. Distilled water was daily administered to rats in the normal group and the model group by gastrogavage. Clomiphene citrate (0. 58 mg/100 g) was daily administered to rats in the WM group for 5 successive days. WYR at 5. 2, 2. 6, 1. 3 mg/100 g was daily administered to rats in high, middle, and low dose WYR groups for 21 successive days. Levels of follicular stimulating hormone (FSH) , luteinizing hormone (LH) , estradiol (E₂) , progesterone (P) , and prolactin (PRL) were detected using radioimmunoassay. Contents of inhibin (INH) , activin (ACT) , and follistatin (FS) were measured using ELISA. Results Compared with the normal group, serum levels of FSH and LH increased, and P level decreased in the model group (P <0. 05) ; INH level decreased and FS level increased in the model group (P<0. 05). Compared with the model group, serum FSH level decreased in the WM group and 3 WYR groups, P level decreased in the WM group (P <0. 05); INH increased and FS levels decreased in the WM group and 3 WYR groups; ACT level increased in the high dose WYR group, with statistical differ- ence (P <0. 05). Conclusion WYR promoted follicular development possibly through regulating INH- ACT-FS system and gonadal hormone level.
目的 探讨温肾养血方（WYR）对无排卵大鼠抑制素-激活素-卵泡抑素（INH-ACT-FS）系统及性腺激素水平的影响。方法 选取76只9日龄大鼠，经颈背部皮下注射丙酸睾酮（每只大鼠1.25 mg/0.05 mL）建立无排卵大鼠模型。将58只造模成功的大鼠按随机数字表法分为5组，即模型组（n =10）、西药组（n =12）、WYR高、中、低剂量组（n =12）。另选取10只22日龄大鼠作为正常组。正常组和模型组大鼠每日经胃管给予蒸馏水。西药组大鼠每日给予枸橼酸氯米芬（0.58 mg/100 g），连续5天。WYR高、中、低剂量组大鼠每日分别给予5.2、2.6、1.3 mg/100 g WYR，连续21天。采用放射免疫法检测卵泡刺激素（FSH）、黄体生成素（LH）、雌二醇（E₂）、孕酮（P）和催乳素（PRL）水平。采用酶联免疫吸附测定法检测抑制素（INH）、激活素（ACT）和卵泡抑素（FS）含量。结果 与正常组比较，模型组血清FSH、LH水平升高，P水平降低（P <0.05）；模型组INH水平降低，FS水平升高（P<0.05）。与模型组比较，西药组和3个WYR组血清FSH水平降低，西药组P水平降低（P <0.05）；西药组和3个WYR组INH升高，FS水平降低；高剂量WYR组ACT水平升高，差异有统计学意义（P <0.05）。结论 WYR可能通过调节INH-ACT-FS系统及性腺激素水平促进卵泡发育。
