Nishimura T, Itoh T
Department of Anatomy, Tohoku University School of Medicine, Sendai, Japan.
Eur J Immunol. 1988 Dec;18(12):2077-80. doi: 10.1002/eji.1830181231.
Normal mouse spleen cells express low levels of lymphocyte function-associated antigen-1 (LFA-1) as well as other lymphoid cells. However, fractionation of spleen cells with Percoll discontinuous gradients resulted in the appearance of lymphocytes expressing high levels of LFA-1 molecule (LFA-1 high lymphocytes) in parallel with the enrichment of natural killer (NK) activity. Lower density spleen cells (fractions 1 and 2) expressed higher level of LFA-1 antigen than unfractionated spleen cells and showed a higher NK activity. In contrast, higher density spleen cells (fractions 3 and 4) expressed lower levels of LFA-1 antigen and revealed lower NK activity. LFA-1 high lymphocytes possessed a high level of asialo GM1, which was the cell surface marker for NK cells. Moreover, sorting of LFA-1 high lymphocytes from spleen cells caused a great enrichment of NK cells. These results demonstrated that in vivo NK cells expressed higher levels of LFA-1 molecule, which was an important adhesion molecule for NK cell-mediated cytotoxicity.
正常小鼠脾细胞与其他淋巴细胞一样，表达低水平的淋巴细胞功能相关抗原-1（LFA-1）。然而，用Percoll不连续梯度对脾细胞进行分级分离，导致出现表达高水平LFA-1分子的淋巴细胞（LFA-1高表达淋巴细胞），同时自然杀伤（NK）活性增强。低密度脾细胞（第1和2组分）比未分级的脾细胞表达更高水平的LFA-1抗原，并且显示出更高的NK活性。相反，高密度脾细胞（第3和4组分）表达较低水平的LFA-1抗原，并且NK活性较低。LFA-1高表达淋巴细胞具有高水平的脱唾液酸GM1，这是NK细胞的细胞表面标志物。此外，从脾细胞中分选LFA-1高表达淋巴细胞导致NK细胞大量富集。这些结果表明，体内NK细胞表达更高水平的LFA-1分子，这是NK细胞介导的细胞毒性的重要黏附分子。
