School of Life Sciences, Guangzhou University, Guangzhou 510655, China; Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China.
School of Life Sciences, Guangzhou University, Guangzhou 510655, China.
Ecotoxicol Environ Saf. 2019 Apr 30;171:579-586. doi: 10.1016/j.ecoenv.2018.12.053. Epub 2019 Jan 15.
Norgestrel (NGT) is a synthetic progestin used in human and veterinary medicine. Adult female mosquitofish were exposed to NGT for 42 d at 377 ng L. The fin morphology and the liver transcriptome were assessed. NGT exposure increased ray 4:6 length ratio. As compared to the control, NGT treatment affected the expression of 11,772 annotated transcripts in female mosquitofish. Specifically, we found 5780 were repressed while 5992 were significantly induced. Gene ontology (GO) analysis showed that 53 KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways and 158 GO terms were significantly over expressed. Genes showing the largest magnitude of expression changes were related to fin development, androgen biosynthesis, and lipid and fatty acid metabolisms, suggesting the involvement of these biological processes in response to NGT exposure in G. affinis. This first comprehensive study on the transcriptomic alterations by NGT in G. affinis not only provides valuable information on the development of molecular markers but also opens new avenues for studies on the molecular mechanisms of effects of NGT in particular and possibly other progestins in G. affinis.
去氧孕烯（NGT）是一种在人类和兽医医学中使用的合成孕激素。成年雌性食蚊鱼在 377ng/L 的浓度下暴露于 NGT 中 42 天。评估了鱼鳍形态和肝脏转录组。NGT 暴露增加了鳍条 4:6 的长度比。与对照组相比，NGT 处理影响了雌性食蚊鱼 11772 个注释转录本的表达。具体来说，我们发现 5780 个被抑制，而 5992 个被显著诱导。基因本体（GO）分析表明，有 53 个京都基因与基因组百科全书（KEGG）途径和 158 个 GO 术语显著过表达。表现出最大表达变化幅度的基因与鳍发育、雄激素生物合成以及脂质和脂肪酸代谢有关，这表明这些生物过程参与了 G. affinis 对 NGT 暴露的反应。这是首次在 G. affinis 中对 NGT 引起的转录组变化进行的全面研究，不仅为开发分子标记提供了有价值的信息，而且为研究 NGT 及其他孕激素在 G. affinis 中的作用的分子机制开辟了新的途径。
