INSERM Unit U1015, Laboratoire de Recherche Translationnelle en Immunothérapie, Gustave Roussy, Villejuif, France; Department of Urology, Hôpital Foch, Université Versailles-Saint-Quentin-en-Yvelines, Université Paris-Saclay, Suresnes, France.
Department of Urology, Hôpital Foch, Université Versailles-Saint-Quentin-en-Yvelines, Université Paris-Saclay, Suresnes, France.
Eur J Cancer. 2019 Feb;108:111-119. doi: 10.1016/j.ejca.2018.12.010. Epub 2019 Jan 14.
Assessment of tumour-infiltrating lymphocytes (TILs) can provide important prognostic information in various cancers and may be of value in predicting response to immunotherapy. The objective of the present study was to investigate the association of stromal lymphocytic infiltration with clinicopathological parameters and their correlation with outcomes in patients with high-grade pT1 non-muscle-invasive bladder cancer (NMIBC).
We retrospectively analysed clinical data and formalin-fixed paraffin-embedded (FFPE) tissues of 147 patients with primary high-grade pT1 NMIBC who underwent transurethral resection of the bladder. The stromal TIL density was scored as percentage of the stromal area infiltrated by mononuclear inflammatory cells over the total intratumoural stromal area. The main end-point was correlation with cancer-specific survival (CSS).
Median follow-up was 8.2 years (6.1-9.5). Induction Bacillus Calmette-Guérin therapy was undergone by 126 patients (86%). Stromal TILs were high (≥10%) in 82 tumours (56%) and were positively associated with the tumour invasion depth (p = 0.01) and cancers with variant histology (p = 0.01). For the CSS analysis, high (≥10%) versus. low (<10%) stromal TIL hazard ratio (95% confidence interval) was 1.70 (0.7-3.9, p = 0.2).
A higher density of stromal TILs was associated with the tumour invasion depth in pT1 NMIBC. The level of TILs was not associated with survival outcomes. These data suggest that tumour aggressiveness is associated with an increased adaptive immune response in pT1 NMIBC. Characterisation of T-cell subtypes along with B-cells may be critical to enhance our knowledge of the host immune response in patients with high-risk NMIBC.
肿瘤浸润淋巴细胞（TILs）的评估可为各种癌症提供重要的预后信息，并且可能对预测免疫治疗反应有价值。本研究的目的是研究间质淋巴细胞浸润与临床病理参数的相关性及其与高分级 pT1 非肌肉浸润性膀胱癌（NMIBC）患者结局的相关性。
我们回顾性分析了 147 例接受经尿道膀胱肿瘤切除术的原发性高分级 pT1 NMIBC 患者的临床数据和福尔马林固定石蜡包埋（FFPE）组织。间质 TIL 密度按单核炎性细胞浸润的间质面积占肿瘤内总间质面积的百分比进行评分。主要终点是与癌症特异性生存（CSS）的相关性。
中位随访时间为 8.2 年（6.1-9.5）。126 例患者（86%）接受了诱导卡介苗治疗。82 例肿瘤（56%）的间质 TIL 较高（≥10%），与肿瘤浸润深度（p=0.01）和具有变异组织学的癌症（p=0.01）呈正相关。在 CSS 分析中，高（≥10%）与低（<10%）间质 TIL 危险比（95%置信区间）为 1.70（0.7-3.9，p=0.2）。
在 pT1 NMIBC 中，较高密度的间质 TIL 与肿瘤浸润深度相关。TIL 水平与生存结果无关。这些数据表明，肿瘤侵袭性与 pT1 NMIBC 中适应性免疫反应的增强有关。T 细胞亚群和 B 细胞的特征可能对增强我们对高危 NMIBC 患者宿主免疫反应的认识至关重要。
