• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

藻蓝蛋白/聚乙二醇-（PG-PEI）减轻肝缺血/再灌注诱导的胰岛损伤，扩大胰岛功能。

Phycocyanin/PEG--(PG--PEI) attenuated hepatic ischemia/reperfusion-induced pancreatic islet injury and enlarged islet functionality.

机构信息

Department of Pathology and Pathophysiology, Provincial Key Discipline of Pharmacology, Jiaxing University Medical College, Jiaxing, Zhejiang Province, PR China,

Department of Pharmaceutical Chemistry, Medical College, Shantou University, Shantou, PR China.

出版信息

Int J Nanomedicine. 2019 Jan 3;14:339-351. doi: 10.2147/IJN.S190938. eCollection 2019.

DOI:10.2147/IJN.S190938
PMID:30655667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6322515/
Abstract

BACKGROUND

Hepatic ischemia/reperfusion-induced pancreatic islet injury (HI/RIPII) was an important pathophysiological phenomenon in clinics. In the present study, we observed the effects of phycocyanin on HI/RIPII. However, the half-life of phycocyanin was extremely short and limited its use in vivo.

MATERIALS AND METHODS

In order to overcome this shortcoming, poly(ethylene glycol)-(poly(l-glutamic acid)--polyethylenimine) (PEG--(PG--PEI)) was synthesized and estimated as a nanocarrier for lengthening delivery of phycocyanin through the abdominal subcutaneous injection in rats. Phycocyanin (isoelectric point=4.3) was encapsulated with PEG--(PG--PEI) via electrostatic interactions at pH 7.4.

RESULTS

In vitro phycocyanin was fast and efficiently encapsulated and showing efficient loading and sustained release. In vivo the anti-HI/RIPII function of phycocyanin/PEG--(PG--PEI) complex was surveyed in rats using free phycocyanin as the controls, and the results showed that phycocyanin/PEG--(PG--PEI) complex reduced HI/RIPII property and enlarged islet functionality.

CONCLUSION

These results suggested that PEG--(PG--PEI) might be treated as a potential phycocyanin nanocarrier.

摘要

背景

肝缺血/再灌注诱导的胰岛损伤（HI/RIPII）是临床中的一个重要病理生理现象。在本研究中，我们观察了藻蓝蛋白对 HI/RIPII 的影响。然而，藻蓝蛋白的半衰期极短，限制了其在体内的应用。

材料和方法

为了克服这一缺点，合成了聚乙二醇-（聚谷氨酸-聚亚乙基亚胺）（PEG-（PG-PEI）），并将其作为通过腹部皮下注射向大鼠体内输送藻蓝蛋白的纳米载体进行了评估。藻蓝蛋白（等电点=4.3）在 pH 7.4 下通过静电相互作用与 PEG-（PG-PEI）结合。

结果

体外藻蓝蛋白快速高效包封，表现出高效负载和持续释放。体内，以游离藻蓝蛋白为对照，研究了藻蓝蛋白/PEG-（PG-PEI）复合物在大鼠中的抗 HI/RIPII 功能，结果表明，藻蓝蛋白/PEG-（PG-PEI）复合物减轻了 HI/RIPII 特性并扩大了胰岛功能。

结论

这些结果表明，PEG-（PG-PEI）可能被视为一种潜在的藻蓝蛋白纳米载体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/d7c653e2b352/ijn-14-339Fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/447ade382fc8/ijn-14-339Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/5fc76ded8234/ijn-14-339Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/cd746502d82d/ijn-14-339Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/dc9bdeed883f/ijn-14-339Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/24177b04f732/ijn-14-339Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/e6b20b9f7d9c/ijn-14-339Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/69231f8a7b68/ijn-14-339Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/afdf14a0e1c0/ijn-14-339Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/09f78af96ebd/ijn-14-339Fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/d7c653e2b352/ijn-14-339Fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/447ade382fc8/ijn-14-339Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/5fc76ded8234/ijn-14-339Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/cd746502d82d/ijn-14-339Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/dc9bdeed883f/ijn-14-339Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/24177b04f732/ijn-14-339Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/e6b20b9f7d9c/ijn-14-339Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/69231f8a7b68/ijn-14-339Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/afdf14a0e1c0/ijn-14-339Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/09f78af96ebd/ijn-14-339Fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c42/6322515/d7c653e2b352/ijn-14-339Fig10.jpg

相似文献

1
Phycocyanin/PEG--(PG--PEI) attenuated hepatic ischemia/reperfusion-induced pancreatic islet injury and enlarged islet functionality.藻蓝蛋白/聚乙二醇-（PG-PEI）减轻肝缺血/再灌注诱导的胰岛损伤，扩大胰岛功能。
Int J Nanomedicine. 2019 Jan 3;14:339-351. doi: 10.2147/IJN.S190938. eCollection 2019.
2
Sustained delivery of insulin-loaded block copolymers: Potential implications on renal ischemia/reperfusion injury in diabetes mellitus.负载胰岛素的嵌段共聚物的持续递送：对糖尿病肾缺血/再灌注损伤的潜在影响。
Biomed Pharmacother. 2017 Jul;91:534-545. doi: 10.1016/j.biopha.2017.04.118. Epub 2017 May 5.
3
Pharmacological Signatures of the Exenatide Nanoparticles Against Hepatic Ischemia/Reperfusion-induced Pancreatic Injury.艾塞那肽纳米颗粒对肝缺血/再灌注诱导的胰腺损伤的药理特征
Transplant Proc. 2019 Apr;51(3):960-965. doi: 10.1016/j.transproceed.2019.01.031. Epub 2019 Jan 9.
4
[Synthesis and characterization of PEG-b-(PG-g-PEI) for gene delivery].用于基因递送的聚乙二醇-b-(聚谷氨酸-g-聚乙烯亚胺)的合成与表征
Nan Fang Yi Ke Da Xue Xue Bao. 2013 Nov;33(11):1643-7.
5
In vitro and in vivo protein release and anti-ischemia/reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(l-lysine) nanoparticles.载骨形态发生蛋白 2 的甘草次酸-聚乙二醇-b-聚赖氨酸纳米粒的体外和体内蛋白释放及抗缺血/再灌注损伤特性。
Int J Nanomedicine. 2017 Oct 17;12:7613-7625. doi: 10.2147/IJN.S146546. eCollection 2017.
6
PEG--(PELG--PLL) nanoparticles as TNF-α nanocarriers: potential cerebral ischemia/reperfusion injury therapeutic applications.聚乙二醇-（聚乙交酯-左旋聚乳酸）纳米颗粒作为肿瘤坏死因子-α纳米载体：潜在的脑缺血/再灌注损伤治疗应用
Int J Nanomedicine. 2017 Mar 23;12:2243-2254. doi: 10.2147/IJN.S130842. eCollection 2017.
7
The effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(l-lysine) block copolymer on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor.负载胰岛素的线性聚乙二醇刷状聚（L-赖氨酸）嵌段共聚物通过下调缺氧诱导因子对肾缺血/再灌注诱导的肺损伤的影响
Int J Nanomedicine. 2016 Apr 27;11:1717-30. doi: 10.2147/IJN.S99890. eCollection 2016.
8
Poly (Ethylene Glycol)-Block-Brush Poly (L-Lysine) Copolymer as an Efficient Nanocarrier for Human Hepatocyte Growth Factor with Enhanced Bioavailability and Anti-Ischemia Reperfusion Injury Efficacy.聚（乙二醇）-嵌段-刷状聚（L-赖氨酸）共聚物作为人肝细胞生长因子的高效纳米载体，具有增强的生物利用度和抗缺血再灌注损伤功效。
Kidney Blood Press Res. 2017;42(3):495-508. doi: 10.1159/000479642. Epub 2017 Aug 30.
9
Brush-shaped polycation with poly(ethylenimine)-b-poly(ethylene glycol) side chains as highly efficient gene delivery vector.具有聚（亚乙基亚胺）-b-聚（乙二醇）侧链的刷状聚阳离子作为高效基因传递载体。
Int J Pharm. 2010 Jun 15;392(1-2):118-26. doi: 10.1016/j.ijpharm.2010.03.043. Epub 2010 Mar 25.
10
Alda-1, an ALDH2 activator, protects against hepatic ischemia/reperfusion injury in rats via inhibition of oxidative stress.Alda-1，一种 ALDH2 激活剂，通过抑制氧化应激来保护大鼠免受肝缺血/再灌注损伤。
Free Radic Res. 2018 Jun;52(6):629-638. doi: 10.1080/10715762.2018.1459042. Epub 2018 Apr 13.

引用本文的文献

1
Evaluation of the hepatoprotective effect of naringenin loaded nanoparticles against acetaminophen overdose toxicity.评价柚皮素纳米粒对乙酰氨基酚过量中毒的肝保护作用。
Drug Deliv. 2022 Dec;29(1):3256-3269. doi: 10.1080/10717544.2022.2139431.
2
Progress of Microencapsulated Phycocyanin in Food and Pharma Industries: A Review.微囊藻蓝蛋白在食品和制药工业中的研究进展：综述。
Molecules. 2022 Sep 9;27(18):5854. doi: 10.3390/molecules27185854.
3
Combined Systemic Intake of K-ATP Opener (Nicorandil) and Mesenchymal Stem Cells Preconditioned With Nicorandil Alleviates Pancreatic Insufficiency in a Model of Bilateral Renal Ischemia/Reperfusion Injury.

本文引用的文献

1
Poly (Ethylene Glycol)-Block-Brush Poly (L-Lysine) Copolymer as an Efficient Nanocarrier for Human Hepatocyte Growth Factor with Enhanced Bioavailability and Anti-Ischemia Reperfusion Injury Efficacy.聚（乙二醇）-嵌段-刷状聚（L-赖氨酸）共聚物作为人肝细胞生长因子的高效纳米载体，具有增强的生物利用度和抗缺血再灌注损伤功效。
Kidney Blood Press Res. 2017;42(3):495-508. doi: 10.1159/000479642. Epub 2017 Aug 30.
2
Overexpression of Brg1 Alleviates Hepatic Ischemia/Reperfusion-Induced Acute Lung Injury through Antioxidative Stress Effects.Brg1 过表达通过抗氧化应激作用减轻肝缺血/再灌注诱导的急性肺损伤。
Oxid Med Cell Longev. 2017;2017:8787392. doi: 10.1155/2017/8787392. Epub 2017 Jul 16.
3
K-ATP开放剂（尼可地尔）与经尼可地尔预处理的间充质干细胞联合全身给药可减轻双侧肾缺血/再灌注损伤模型中的胰腺功能不全。
Front Physiol. 2022 Jun 23;13:934597. doi: 10.3389/fphys.2022.934597. eCollection 2022.
4
Phycobiliproteins: Structural aspects, functional characteristics, and biotechnological perspectives.藻胆蛋白：结构方面、功能特性及生物技术前景
Comput Struct Biotechnol J. 2022 Feb 23;20:1506-1527. doi: 10.1016/j.csbj.2022.02.016. eCollection 2022.
Acinar-to-Ductal Metaplasia Induced by Transforming Growth Factor Beta Facilitates KRAS-driven Pancreatic Tumorigenesis.
转化生长因子β诱导的腺泡-导管化生促进KRAS驱动的胰腺肿瘤发生。
Cell Mol Gastroenterol Hepatol. 2017 May 31;4(2):263-282. doi: 10.1016/j.jcmgh.2017.05.005. eCollection 2017 Sep.
4
Preparation of exenatide-loaded linear poly(ethylene glycol)-brush poly(l-lysine) block copolymer: potential implications on diabetic nephropathy.负载艾塞那肽的线性聚乙二醇-刷状聚（L-赖氨酸）嵌段共聚物的制备：对糖尿病肾病的潜在影响
Int J Nanomedicine. 2017 Jun 29;12:4663-4678. doi: 10.2147/IJN.S136646. eCollection 2017.
5
Hepatic ischemia/reperfusion injury disrupts the homeostasis of kidney primary cilia via oxidative stress.肝缺血/再灌注损伤通过氧化应激破坏肾脏初级纤毛的内稳态。
Biochim Biophys Acta Mol Basis Dis. 2017 Jul;1863(7):1817-1828. doi: 10.1016/j.bbadis.2017.05.004. Epub 2017 May 8.
6
Sustained delivery of insulin-loaded block copolymers: Potential implications on renal ischemia/reperfusion injury in diabetes mellitus.负载胰岛素的嵌段共聚物的持续递送：对糖尿病肾缺血/再灌注损伤的潜在影响。
Biomed Pharmacother. 2017 Jul;91:534-545. doi: 10.1016/j.biopha.2017.04.118. Epub 2017 May 5.
7
Simvastatin nanoparticles attenuated intestinal ischemia/reperfusion injury by downregulating BMP4/COX-2 pathway in rats.辛伐他汀纳米颗粒通过下调大鼠BMP4/COX-2通路减轻肠道缺血/再灌注损伤。
Int J Nanomedicine. 2017 Mar 29;12:2477-2488. doi: 10.2147/IJN.S126063. eCollection 2017.
8
Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice.干扰素调节因子-1 通过 P38/P62 通路激活自噬加重小鼠肝缺血再灌注损伤。
Sci Rep. 2017 Mar 7;7:43684. doi: 10.1038/srep43684.
9
Quantifying ROS levels using CM-HDCFDA and HyPer.使用CM-HDCFDA和HyPer定量活性氧水平。
Methods. 2016 Oct 15;109:3-11. doi: 10.1016/j.ymeth.2016.06.008. Epub 2016 Jun 11.
10
The effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(l-lysine) block copolymer on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor.负载胰岛素的线性聚乙二醇刷状聚（L-赖氨酸）嵌段共聚物通过下调缺氧诱导因子对肾缺血/再灌注诱导的肺损伤的影响
Int J Nanomedicine. 2016 Apr 27;11:1717-30. doi: 10.2147/IJN.S99890. eCollection 2016.