Ge Yazhong, Gao Qing, Yan Fengjiao, Zhang Xiaona, Liu Yurong, Zhou Yi
Department of Healthy Food Development, Infinitus (China) Company Ltd., Guangzhou, Guangdong 510024, P.R. China.
School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510102, P.R. China.
Oncol Lett. 2019 Jan;17(1):422-431. doi: 10.3892/ol.2018.9625. Epub 2018 Oct 26.
Su Yang Decoction (SYD) is a popular healthcare product comprised of various brassicaceous vegetables known to exhibit anticancer activity. However, the effects of SYD on tumor growth, following its combination with brassicaceous vegetables into a compound formula, require further investigation. In the present study, a quality control of SYD was subjected to high-performance liquid chromatography for the quantitative and qualitative determination of sulforaphane, the main anticancer component of SYD. SYD inhibited colon cancer cell proliferation in a dose- and time-dependent manner and induced G1 phase arrest in colon cancer HT-29 cell lines. In addition, SYD triggered caspase-mediated apoptosis in a dose-dependent manner and induced the cleavage of poly (ADP-ribose) polymerase, tumor necrosis factor superfamily member 10, X-linked inhibitor of apoptosis, and truncated BH3 interacting domain death agonist. Furthermore, the expression of FADD-like interleukin-1β-converting enzyme (FLICE)-like inhibitory proteins (FLIPs) and long isoform of FLICE-inhibitory protein was reduced by SYD and the direct targeting of cellular-FLIP with small interfering RNA inhibited colon cancer cell proliferation and decreased the SYD concentration required for proliferation inhibition. SYD treatment was also associated with the translocation of proapoptotic BCL2 associated X, apoptosis regulator to the mitochondria and the release of cytochrome from the mitochondria to the cytosol. The aforementioned results indicate that SYD exerts anti-colorectal cancer effects through an underlying mechanism that may involve caspase activation.
苏阳汤（SYD）是一种由多种已知具有抗癌活性的十字花科蔬菜组成的流行保健品。然而，SYD与十字花科蔬菜组合成复方后对肿瘤生长的影响仍需进一步研究。在本研究中，对SYD进行了质量控制，采用高效液相色谱法定量和定性测定SYD的主要抗癌成分萝卜硫素。SYD以剂量和时间依赖性方式抑制结肠癌细胞增殖，并诱导结肠癌HT-29细胞系G1期阻滞。此外，SYD以剂量依赖性方式触发半胱天冬酶介导的凋亡，并诱导聚（ADP-核糖）聚合酶、肿瘤坏死因子超家族成员10、凋亡抑制蛋白X连锁抑制剂和截短的BH3相互作用域死亡激动剂的裂解。此外，SYD降低了FADD样白细胞介素-1β转换酶（FLICE）样抑制蛋白（FLIPs）和FLICE抑制蛋白长异构体的表达，用小干扰RNA直接靶向细胞FLIP可抑制结肠癌细胞增殖并降低增殖抑制所需的SYD浓度。SYD处理还与促凋亡蛋白BCL2相关X（凋亡调节因子）转位至线粒体以及细胞色素从线粒体释放至胞质溶胶有关。上述结果表明，SYD通过可能涉及半胱天冬酶激活的潜在机制发挥抗结直肠癌作用。
