Hu Guozhang, Fang Wei, Liu Naijie, Li Chang
Department of Emergency Medicine, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
Ward 1, Department of Neurosurgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
Oncol Lett. 2019 Jan;17(1):891-896. doi: 10.3892/ol.2018.9651. Epub 2018 Nov 1.
Effects of mir-128a on the proliferation and migration of human glioma U251 cells were explored. The constructed mir-128a-shRNA lentivirus vector (infection group) and scramble shRNA (interference group) were transfected into glioma U251 cells, and uninfected U251 cells as control group. The expression level of mir-128a, the ability of proliferation, invasion, apoptosis and migration of cells in each group were detected by RT-qPCR, MTT assay, Transwell migration , cell wound scratch assay and TUNEL cell apoptosis assay. The expression level of mir-128a in U251 cells of infection group was significantly higher than that in U251 cells of interference group (P<0.05). Τhe expression level of mir-128a in U251 cells of control group was significantly lower than that in U251 cells of infection group (P<0.05). The OD values of infection and control group were lower than that of interference group at 6, 12, 24, 48 and 72 h, and the OD values of infection were lower than that of control group at 6, 12, 24, 48 and 72 h (P<0.05). Compared with infection and control group, the number of membrane-penetrating cells in U251 cells of interference group increased significantly (P<0.05). The apoptosis rate of U251 cells of infection and control group was significantly higher than that of interference group, and the apoptosis rate of infection was significantly higher than that of control group (P<0.05). The migration distance of U251 cells of infection and interference group was significantly larger than that of control group (P<0.05). Τhe migration distance of U251 cells of interference group was significantly larger than that of infection group (P<0.05). mir-128a may play a role similar to anti-oncogene in glioma, inhibiting the ability of proliferation, invasion and migration of glioma cells, and promoting the apoptosis of glioma cells.
探讨了mir-128a对人胶质瘤U251细胞增殖和迁移的影响。将构建的mir-128a-shRNA慢病毒载体（感染组）和乱序shRNA（干扰组）转染至胶质瘤U251细胞，未感染的U251细胞作为对照组。通过RT-qPCR、MTT法、Transwell迁移实验、细胞划痕实验和TUNEL细胞凋亡检测法检测各组中mir-128a的表达水平、细胞的增殖、侵袭、凋亡及迁移能力。感染组U251细胞中mir-128a的表达水平显著高于干扰组U251细胞（P<0.05）。对照组U251细胞中mir-128a的表达水平显著低于感染组U251细胞（P<0.05）。感染组和对照组在6、12、24、48和72小时的OD值均低于干扰组，且感染组在6、12、24、48和72小时的OD值低于对照组（P<0.05）。与感染组和对照组相比，干扰组U251细胞中穿膜细胞数量显著增加（P<0.05）。感染组和对照组U251细胞的凋亡率显著高于干扰组，且感染组的凋亡率显著高于对照组（P<0.05）。感染组和干扰组U251细胞的迁移距离显著大于对照组（P<0.05）。干扰组U251细胞的迁移距离显著大于感染组（P<0.05）。mir-128a在胶质瘤中可能发挥类似于抑癌基因的作用，抑制胶质瘤细胞的增殖、侵袭和迁移能力，并促进胶质瘤细胞的凋亡。
