Liu Tao, Wei Rendong, Zhang Yiting, Chen Wen, Liu Haidong
Department of Hepatology, The Sixth People's Hospital of Qingdao, Qingdao, Shandong 266033, P.R. China.
Department of Infectious Diseases, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.
Oncol Lett. 2019 Jan;17(1):1030-1034. doi: 10.3892/ol.2018.9640. Epub 2018 Oct 30.
Association between the expression of nuclear factor κB (NF-κB) and the drug resistance of hepatoma cells was investigated. HepG-2 cells and HepG2/ADM cells were cultured, respectively. The morphology and status of the two groups of cells were observed by cell white light. The immunofluorescence by NF-κB and MDR1 staining on HepG-2 cells and HepG2/ADM cells, respectively, was applied and the fluorescence expression in the two groups of cells was observed. RT-qPCR was used to detect the expression of NF-κB and MDR1 mRNA, the NF-κB and MDR1 protein expression was detected by western blot analysis. The results of cell white illumination showed that the structure of HepG-2 and HepG2/ADM cells was complete and the cell morphology was normal, and there was no significant difference, and could be used for comparative study. Immunofluorescence staining showed that the expression of NF-κB and MDR1 in HepG-2 cells was very low, while the expression of NF-κB and MDR1 in HepG2/ADM cells was increased significantly. The RT-qPCR results showed that NF-κB and MDR1 mRNA expression in HepG-2 cells was very low, while NF-κB and MDR1 mRNA expression in HepG-2/ADM cells was significantly increased, and western blot results showed that NF-κB and MDR1 protein expression in HepG-2 cells was very low, while NF-κB and MDR1 protein expression in HepG-2/ADM cells was increased significantly. The results of variance analysis showed that there was significant difference in the expression of the control group and paeonol group (P<0.01). In conclusion, the expression of NF-κB in the drug-resistant cells of liver cancer is closely related to the resistance-related gene . This result may provide a new solution for the drug resistance of liver cancer.
研究了核因子κB（NF-κB）表达与肝癌细胞耐药性之间的关联。分别培养HepG-2细胞和HepG2/ADM细胞。通过细胞白光观察两组细胞的形态和状态。分别对HepG-2细胞和HepG2/ADM细胞进行NF-κB和MDR1染色的免疫荧光检测，并观察两组细胞中的荧光表达。采用RT-qPCR检测NF-κB和MDR1 mRNA的表达，通过蛋白质免疫印迹分析检测NF-κB和MDR1蛋白的表达。细胞白光观察结果显示，HepG-2细胞和HepG2/ADM细胞结构完整，细胞形态正常，且无显著差异，可用于对比研究。免疫荧光染色显示，HepG-2细胞中NF-κB和MDR1的表达非常低，而HepG2/ADM细胞中NF-κB和MDR1的表达显著增加。RT-qPCR结果显示，HepG-2细胞中NF-κB和MDR1 mRNA表达非常低，而HepG-2/ADM细胞中NF-κB和MDR1 mRNA表达显著增加，蛋白质免疫印迹结果显示，HepG-2细胞中NF-κB和MDR1蛋白表达非常低，而HepG-2/ADM细胞中NF-κB和MDR1蛋白表达显著增加。方差分析结果显示，对照组和丹皮酚组的表达存在显著差异（P<0.01）。综上所述，肝癌耐药细胞中NF-κB的表达与耐药相关基因密切相关。这一结果可能为肝癌耐药问题提供新的解决办法。
