Fujiwara Kazuki, Fujikawa Takashi, Kawakami Akira, Sonoda Ryoichi, Miyasaka Atsushi
Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Suya 2421, Koshi, Kumamoto 861-1192, Japan.
Institute of Fruit Tree and Tea Science, NARO, Fujimoto 2-1, Tsukuba, Ibaraki 305-8605, Japan.
Data Brief. 2018 Dec 18;22:693-696. doi: 10.1016/j.dib.2018.12.039. eCollection 2019 Feb.
(Berk.) is an important biotrophic oomycete that causes downy mildew on onion ( L.) worldwide, especially in humid and temperate regions. The disease attacks bulb and seed production of onion, resulting in losses in yield and quality of bulbs. Epidemiological studies have increased our understanding and control of downy mildew on onion; however, little is known about the molecular aspects of behavior during infection. Here, we isolated RNA from four samples of sporangia and sporangiophores of , which were maintained by spore inoculation onto onions in a growth chamber. We then used an Ion PGM next generation sequencer to acquire and assemble the RNA sequences of . By transcriptome shotgun assembly, we obtained 2335 contigs (N50, 884 nucleotides (nt); mean length, 881.6 nt). The data are accessible at NCBI (BioProject PRJNA391849). Our data resource will facilitate further studies of the molecular events during infection.
(伯克氏菌)是一种重要的活体营养卵菌,在全球范围内导致洋葱(L.)霜霉病,尤其是在潮湿和温带地区。这种病害侵袭洋葱的鳞茎和种子生产,导致鳞茎产量和品质下降。流行病学研究增进了我们对洋葱霜霉病的理解和控制;然而,对于其在感染过程中行为的分子层面知之甚少。在此,我们从通过在生长室中将孢子接种到洋葱上而维持的四个样本的游动孢子囊和孢囊梗中分离了RNA。然后我们使用Ion PGM下一代测序仪来获取和组装(该菌)的RNA序列。通过转录组鸟枪法组装,我们获得了2335个重叠群(N50,884个核苷酸(nt);平均长度,881.6 nt)。数据可在NCBI(生物项目PRJNA391849)获取。我们的数据资源将有助于进一步研究(该菌)感染期间的分子事件。