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两个对比水稻品种在干旱胁迫和恢复处理下的无标记和等压串联质量标签(TMT)多重定量蛋白质组学数据

Label-free and isobaric tandem mass tag (TMT) multiplexed quantitative proteomic data of two contrasting rice cultivars exposed to drought stress and recovery.

作者信息

Wu Yunqi, Mirzaei Mehdi, Atwell Brian J, Haynes Paul A

机构信息

Department of Molecular Sciences, Macquarie University, North Ryde, NSW 2109, Australia.

Department of Biological Sciences, Macquarie University, North Ryde, NSW 2109, Australia.

出版信息

Data Brief. 2018 Dec 15;22:697-702. doi: 10.1016/j.dib.2018.12.041. eCollection 2019 Feb.

Abstract

Two rice cultivars, IAC1131 (drought tolerant) and Nipponbare (drought sensitive), with contrasting genetic backgrounds and levels of tolerance to drought, were analysed using both label-free and tandem mass tags (TMTs) quantitative proteomics approaches, aiming to elucidate the mechanisms of drought tolerance. Four-week-old seedlings of both cultivars were grown in large soil volumes in the glasshouse under controlled conditions and then exposed to moderate and extreme drought for 7 days, followed by 3 days of re-watering period. Mature leaves were harvested from plants of each treatment for protein extraction and subsequent complementary shotgun proteomic analyses. The data from this study are related to the research article "Quantitative proteomic analysis of two different rice varieties reveals that drought tolerance is correlated with reduced abundance of photosynthetic machinery and increased abundance of ClpD1 protease" (Wu et al., 2016) [1].

摘要

使用无标记和串联质量标签(TMT)定量蛋白质组学方法,对两个具有不同遗传背景和耐旱水平的水稻品种IAC1131(耐旱)和日本晴(干旱敏感)进行了分析,旨在阐明耐旱机制。两个品种的四周龄幼苗在温室中可控条件下于大体积土壤中生长,然后分别进行中度和极端干旱处理7天,随后复水3天。从每个处理的植株上收获成熟叶片用于蛋白质提取及后续的互补鸟枪法蛋白质组分析。本研究的数据与研究论文《两种不同水稻品种的定量蛋白质组分析表明,耐旱性与光合机构丰度降低和ClpD1蛋白酶丰度增加相关》(Wu等人,2016年)[1]相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4078/6329202/976e3cf19822/gr1.jpg

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