Department of Molecular Sciences, Macquarie University, North Ryde, NSW, 2109, Australia.
Australian Proteome Analysis Facility, Macquarie University, North Ryde, NSW, 2109, Australia.
Proteomics. 2019 May;19(9):e1800310. doi: 10.1002/pmic.201800310. Epub 2019 Apr 18.
Plants require a distinctive cohort of enzymes to coordinate cell division and expansion. Proteomic analysis now enables interrogation of immature leaf bases where these processes occur. Hence, proteins in tissues sampled from leaves of a drought-tolerant rice (IAC1131) are investigated to provide insights into the effect of soil drying on gene expression relative to the drought-sensitive genotype Nipponbare. Shoot growth zones are dissected to estimate the proportion of dividing cells and extract protein for subsequent tandem mass tags quantitative proteomic analysis. Gene ontology annotations of differentially expressed proteins provide insights into responses of Nipponbare and IAC1131 to drought. Soil drying does not affect the percentage of mitotic cells in IAC1131. More than 800 proteins across most functional categories increase in drought (and decrease on rewatering) in IAC1131, including proteins involved in "organizing the meristem" and "new cell formation". On the other hand, the percentage of dividing cells in Nipponbare is severely impaired during drought and fewer than 200 proteins respond in abundance when growing zones undergo a drying cycle. Remarkably, the proteomes of the growing zones of each genotype respond in a highly distinctive manner, reflecting their contrasting drought tolerance even at the earliest stages of leaf development.
植物需要一组独特的酶来协调细胞分裂和扩张。蛋白质组学分析现在可以研究这些过程发生的未成熟叶片基部。因此，对耐旱水稻（IAC1131）叶片组织中蛋白质进行研究，以深入了解土壤干燥对基因表达的影响，与耐旱基因型日本晴相比。将茎生长区分离出来，以估计分裂细胞的比例，并提取蛋白质进行随后的串联质量标签定量蛋白质组学分析。差异表达蛋白的基因本体注释为日本晴和 IAC1131 对干旱的反应提供了深入的了解。土壤干燥不会影响 IAC1131 中有丝分裂细胞的百分比。在 IAC1131 中，超过 800 种蛋白质在大多数功能类别中增加（在重新浇水时减少），包括参与“组织分生组织”和“新细胞形成”的蛋白质。另一方面，日本晴在干旱期间有丝分裂细胞的百分比严重受损，当生长区经历干燥循环时，只有不到 200 种蛋白质的丰度发生响应。值得注意的是，每个基因型生长区的蛋白质组以高度独特的方式响应，反映了它们在叶片发育的早期阶段就具有不同的耐旱性。
