Wang Yuanyuan, Guo Xiaofang, Peng Honghong, Lu Yunlan, Zeng Xucan, Dai Ke, Zuo Shuqing, Zhou Hongning, Zhang Jiusong, Tong Yigang
Anhui Medical University, Hefei, 230000, China.
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China.
Arch Virol. 2019 Mar;164(3):907-911. doi: 10.1007/s00705-018-04133-5. Epub 2019 Jan 17.
A novel negevirus, tentatively named Manglie virus (MaV), was isolated from Culex tritaeniorhynchus from the village of Manglie, Yunnan, China, in August 2011. It was identified by high-throughput sequencing of cell culture supernatants, and the complete genome was sequenced using an Illumina MiSeq sequencer. The complete MaV genome comprised 9,218 nt encoding three hypothetical proteins and had a poly(A) tail. BLASTn analysis showed that the genome had the greatest similarity to Ngewotan virus strain Nepal22, with query coverage of 100% and 79% identity. Genomic and phylogenetic analyses demonstrated that MaV should be considered a novel negevirus.
2011年8月，从中国云南省芒烈村的三带喙库蚊中分离出一种新型内盖夫病毒，暂命名为芒烈病毒（MaV）。通过对细胞培养上清液进行高通量测序对其进行鉴定，并使用Illumina MiSeq测序仪对完整基因组进行测序。芒烈病毒完整基因组由9218个核苷酸组成，编码三种假定蛋白，并具有一个聚腺苷酸尾。BLASTn分析表明，该基因组与内格沃坦病毒株Nepal22的相似性最高，查询覆盖率为100%，同一性为79%。基因组和系统发育分析表明，芒烈病毒应被视为一种新型内盖夫病毒。
Zotero 插件