Nunes Marcio R T, Contreras-Gutierrez María Angélica, Guzman Hilda, Martins Livia C, Barbirato Mayla Feitoza, Savit Chelsea, Balta Victoria, Uribe Sandra, Vivero Rafael, Suaza Juan David, Oliveira Hamilton, Nunes Neto Joaquin P, Carvalho Valeria L, da Silva Sandro Patroca, Cardoso Jedson F, de Oliveira Rodrigo Santo, da Silva Lemos Poliana, Wood Thomas G, Widen Steven G, Vasconcelos Pedro F C, Fish Durland, Vasilakis Nikos, Tesh Robert B
Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil.
Programa de Estudio y Control de Enfermedades Tropicales - PECET - SIU-Sede de Investigación Universitaria - Universidad de Antioquia, Medellín, Colombia; Grupo de Investigación en Sistemática Molecular-GSM, Facultad de Ciencias,Ciencias, Universidad Nacional de Colombia, sede Medellín, Medellín, Colombia.
Virology. 2017 Apr;504:152-167. doi: 10.1016/j.virol.2017.01.022. Epub 2017 Feb 11.
The recently described taxon Negevirus is comprised of a diverse group of insect-specific viruses isolated from mosquitoes and phlebotomine sandflies. In this study, a comprehensive genetic characterization, molecular, epidemiological and evolutionary analyses were conducted on nearly full-length sequences of 91 new negevirus isolates obtained in Brazil, Colombia, Peru, Panama, USA and Nepal. We demonstrated that these arthropod restricted viruses are clustered in two major phylogenetic groups with origins related to three plant virus genera (Cilevirus, Higrevirus and Blunevirus). Molecular analyses demonstrated that specific host correlations are not present with most negeviruses; instead, high genetic variability, wide host-range, and cross-species transmission were noted. The data presented here also revealed the existence of five novel insect-specific viruses falling into two arthropod-restrictive virus taxa, previously proposed as distinct genera, designated Nelorpivirus and Sandewavirus. Our results provide a better understanding of the molecular epidemiology, evolution, taxonomy and stability of this group of insect-restricted viruses.
最近描述的内盖夫病毒属由从蚊子和白蛉中分离出的多种昆虫特异性病毒组成。在本研究中，对在巴西、哥伦比亚、秘鲁、巴拿马、美国和尼泊尔获得的91株新内盖夫病毒分离株的近全长序列进行了全面的基因特征分析、分子、流行病学和进化分析。我们证明，这些节肢动物限制性病毒聚集在两个主要的系统发育组中，其起源与三个植物病毒属（柑橘病毒属、希格雷病毒属和布鲁内病毒属）有关。分子分析表明，大多数内盖夫病毒不存在特定的宿主相关性；相反，观察到高遗传变异性、广泛的宿主范围和跨物种传播。这里提供的数据还揭示了存在五种新的昆虫特异性病毒，它们属于两个节肢动物限制性病毒分类群，先前被提议为不同的属，分别命名为尼罗皮病毒属和桑德瓦病毒属。我们的结果为更好地理解这组昆虫限制性病毒的分子流行病学、进化、分类学和稳定性提供了依据。
