Batta-Lona Paola Gabriela, Galindo-Sánchez Clara E, Arteaga Maria Clara, Robles-Flores Javier, Jiménez-Rosenberg Sylvia Patricia Aldeheid
a Centro de Educación Científica y de Educación Superior de Ensenada (CICESE) , Ensenada , Mexico.
b Instituto Politécnico Nacional-CICIMAR , La Paz , Mexico.
Mitochondrial DNA A DNA Mapp Seq Anal. 2019 Mar;30(2):375-383. doi: 10.1080/24701394.2018.1538364. Epub 2019 Jan 18.
Accurate identification of fish larval stages is complicated and time-consuming due to the lack of diagnostic morphological characters, especially during early developmental stages. The distribution of lanternfish (Myctophidae) has been described based on the morphological identification of adult stages. Larvae of only a few species of Myctophidae have been described, and the description is not always precise. In this study, larvae were collected and morphologically identified as Diaphus mollis, Hygophum hygomii, H. reinhardtii, H. taaningi, Myctophum obtusirostre and M. selenops. The DNA barcode region of the mitochondrial cytochrome oxidase I gene (COI) was determined for all larvae. The COI sequences matched reference barcodes available in GenBank for 14 of the identified larvae. The remaining COI sequences matched reference barcodes for different species of Myctophidae including Centrobranchus nigroocellatus, Diogenichthys atlanticus and Lepidophanes guentheri. This effort demonstrated the importance of integrated morphological and molecular analysis of species diversity and distribution of the Myctophidae in the Gulf of México.
由于缺乏诊断性形态特征，准确识别鱼类幼体阶段既复杂又耗时，尤其是在发育早期阶段。灯笼鱼（灯笼鱼科）的分布是基于成体阶段的形态识别来描述的。仅描述了少数几种灯笼鱼科鱼类的幼体，而且描述并不总是准确的。在本研究中，收集了幼体并通过形态学鉴定为软腹钻光鱼、光裸眶灯鱼、雷氏眶灯鱼、坦氏眶灯鱼、钝吻烛光鱼和月烛光鱼。测定了所有幼体线粒体细胞色素氧化酶I基因（COI）的DNA条形码区域。14条已鉴定幼体的COI序列与GenBank中可用的参考条形码匹配。其余的COI序列与灯笼鱼科不同物种的参考条形码匹配，包括黑眼中央灯鱼、大西洋长鳍灯鱼和贡氏犀光鱼。这项工作证明了对墨西哥湾灯笼鱼科物种多样性和分布进行形态学与分子分析相结合的重要性。
